Development and high-throughput genotyping of substitution lines carting the chromosome segments of indica 9311 in the background of japonica Nipponbare  

作　　者：Hua Zhang Qiang Zhao Zhi-Zhong Sun Chang-Quan Zhang Qi Feng Shu-Zhu Tang Guo-Hua Liang Ming-Hong Gu Bin Han Qiao-Quan Liu 

机构地区：[1]Jiangsu Key Laboratory of Crop Genetics and Physiology~Key Laboratory of the Ministry of Education for Plant Functional Genomics, College of Agriculture, Yangzhou University, Yangzhou 225009, China [2]National Center for Gene Research, Institute of Plant Physiology and Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China [3]National Center for Plant Gene Research （Shanghai）, Shanghai 200032, China

出　　处：《Journal of Genetics and Genomics》2011年第12期603-611,共9页遗传学报（英文版）

基　　金：supported in part by the National Basic Research Program(Nos.2012CB944803 and 2011CB 100202);the National Natural Science Foundation(No.31071383);the National Special Program for Transgenic Research(No. 2009ZX08009-008B);the Priority Academic Program Development from Jiangsu Government of China

摘　　要：Chromosome segment substitution lines（CSSLs） are useful for the precise mapping of quantitative trait loci（QTLs） and dissection of the genetic basis of complex traits.In this study,two whole-genome sequenced rice cultivars,the japonica Nipponbare and indica 9311 were used as recipient and donor,respectively.A population with 57 CSSLs was developed after crossing and back-crossing assisted by molecular markers, and genotypes were identified using a high-throughput resequencing strategy.Detailed graphical genotypes of 38 lines were constructed based on resequencing data.These CSSLs had a total of 95 substituted segments derived from indica 9311,with an average of about 2.5 segments per CSSL and eight segments per chromosome,and covered about 87.4%of the rice whole genome.A multiple linear regression QTL analysis mapped four QTLs for 1000-grain weight.The largest-effect QTL was located in a region on chromosome 5 that contained a cloned major QTL GW5/qSW5 for grain size in rice.These CSSLs with a background of Nipponbare may provide powerful tools for future whole-genomeChromosome segment substitution lines（CSSLs） are useful for the precise mapping of quantitative trait loci（QTLs） and dissection of the genetic basis of complex traits.In this study,two whole-genome sequenced rice cultivars,the japonica Nipponbare and indica 9311 were used as recipient and donor,respectively.A population with 57 CSSLs was developed after crossing and back-crossing assisted by molecular markers, and genotypes were identified using a high-throughput resequencing strategy.Detailed graphical genotypes of 38 lines were constructed based on resequencing data.These CSSLs had a total of 95 substituted segments derived from indica 9311,with an average of about 2.5 segments per CSSL and eight segments per chromosome,and covered about 87.4%of the rice whole genome.A multiple linear regression QTL analysis mapped four QTLs for 1000-grain weight.The largest-effect QTL was located in a region on chromosome 5 that contained a cloned major QTL GW5/qSW5 for grain size in rice.These CSSLs with a background of Nipponbare may provide powerful tools for future whole-genome

关 键 词：Oryza sativa L. Chromosome segment substitution lines（CSSLs） Molecular marker-assisted selection High-throughput resequencing 

分 类 号：S511.2[农业科学—作物学] Q943[生物学—植物学]