浙江省非O1、非O139群霍乱弧菌快速检测与毒力基因研究  

Rapid detection of non O1 and non O139 Vibrio cholerae and their virulence genes in Zhejiang province

作　　者：罗芸[1] 叶菊莲[1] 金大智[1] 张政[1] 王复甦[1] 徐宝祥[1] 

机构地区：[1]浙江省疾病预防控制中心,浙江杭州310051

出　　处：《疾病监测》2011年第12期935-938,共4页Disease Surveillance

摘　　要：目的建立一种快速检测非O1、非O139群霍乱弧菌方法,并对浙江省部分地区海水产品中非O1、非O139群霍乱弧菌及其携带毒力基因开展快速检测。方法采用三糖斜面、氧化酶实验、粘丝实验、无盐胨水等简单生化进行初筛,对全部结果阳性的菌株用聚合酶链反应(PCR)方法检测霍乱弧菌外膜蛋白OmpW以及毒力基因ctxA、hlyA和toxR。结果按照方法用三糖斜面初筛后共有504份样本阳性,其中氧化酶、粘丝和无盐胨水全部为阳性的有434份;有388份样本OmpW PCR结果为阳性,其中ctxA结果均为阴性,toxR阳性hlyA阳性的有225株。结论建立的快速检测方法提高了非O1、非O139群霍乱弧菌的检出率,缩短了检测时间,节约了检测成本。浙江省非O1、非O139群霍乱弧菌在海水产品中广泛存在,应对其致病性提高警惕,加强监测。Objective To establish a rapid detection assay to detect non O1 and non-O139 Vibrio cholerae in sea foods and their virulence genes in Zhejiang province.Methods Non O1 and non O139 Vibrio cholerae strains were detected by using triple sugar tube,oxidase test,ropiness test and saltless peptone water test,then OmpW,ctxA、hlyA and toxR genes of Vibrio cholerae were detected by PCR assay.Results Totally 504 sample were positive by using triple sugar tube,in addition,434 samples were positive in other 3 tests.Totally 388 strains were OmpW gene positive,but ctxA gene negative and 225 strains were both hlyA and toxR gene positive.Conclusion The established rapid assay could increase the detection rate of non O1 and non O139 Vibrio cholerae,shorten the detection time and reduce test cost.Non O1 and non-O139 Vibrio cholerae generally exist in sea foods in Zhejiang province.It is important to fully understand their pathogenicities and strengthen the disease surveillance.

关 键 词：霍乱弧菌 非O1、非O139群 快速检测 毒力基因 

分 类 号：R378.3[医药卫生—病原生物学]