宁夏地区汉族代谢综合征患者脂联素基因多态性与冠心病的相关性研究  

Study on the correlation of adiponectin gene polymorphism and coronary heart disease in the patients with metabolic syndrome in Han nationality population in Ningxia

作　　者：陈芳[1] 武海亮[1] 杨俏玲[1] 刘雅娟[1] 王洁[1] 杨锐英[1] 

机构地区：[1]银川市第一人民医院心血管内科,宁夏银川750001

出　　处：《中国急救医学》2012年第1期24-29,共6页Chinese Journal of Critical Care Medicine

基　　金：宁夏自然科学基金项目（No.NZ09109）

摘　　要：目的探讨宁夏地区汉族代谢综合征（MS）患者脂联素（APN）水平及其单核苷酸基因多态性（SNP）＋45T／G和＋276G／T两个位点与冠心病（CHD）的关系。方法302例研究对象分为正常对照组、代谢综合征（MS）组、代谢综合征合并冠心病（M＋C）组，采用实验一对照研究方法，应用酶联免疫吸附法（ELISA）及聚合酶链反应一限制性片段长度多态性（PCR—RFLP）技术，对血浆APN水平及APN两个位点基因多态性进行检测。结果与对照组相比，MS组及M＋C组血浆APN水平降低（P〈0．05）；与对照组及MS组相比，M＋C组SNP＋45TG＋GG基因型及G等位基因的分布频率明显降低（P〈0．05）。多因素逐步Logistic回归分析显示，SNP＋276基因多态性为MS危险因素，SNP＋45G等位基因为CHD的保护因素。结论MS患者APN水平降低，携带SNP＋276T等位基因者患MS的风险增加，携带SNP＋45G等位基因的MS患者发生CHD的危险性减小。Objective To investigate the correlation of adiponectin gene polymorphism and coronary heart disease （CHD） in the patients with metabolic syndrome （MS） in Han nationality population in Ningxia. Methods 302 patients were divided into normal control group, MS group and both MS and CHD（ M ＋ C） group. According to experimental -control study, the technique of enzyme - linked immunosorbent assay （ELISA） and polymerase chain reaction - restriction fragment length polymorphism （PCR - RELP） were used to detect concentration of the plasma adiponection and polymorphisms of the two genes of adiponectin. Results Compared with the control group, APN in MS and M ＋ C group reduced （P 〈 0. 05 ）. Compared with the control and MS groups, the genotype frequency of M ＋ C group on SNP ＋ 45TG ＋ GG genotype and G allele went down （ P 〈 0. 05 ）. Multiple factor stepwise Logistic regression analysis showed that SNP ＋ 276 gene polymorphism were risk factors for MS, SNP ＋ 45G allele might be the protective factors for the CHD. Conclusion APN level in the patients with MS declines. People with the SNP ＋ 276T allele are most at risk from MS. SNP ＋ 45G allele may be the protective factors for MS, so people with the SNPS ＋ 45G allele have decreased occurrence of CHD.

关 键 词：脂联素 冠心病 代谢综合征 基因多态性 

分 类 号：R725.8[医药卫生—儿科]