葎草的薄层色谱鉴别研究  

Thin-Layer Chromatography of Herba Humuli Scandens

作　　者：郭沛琳[1] 马逾英[1] 米小琴[1] 卢晓琳[1] 

机构地区：[1]成都中医药大学药学院,四川成都610075

出　　处：《成都中医药大学学报》2011年第4期78-80,共3页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：四川省科技厅基金项目资助(编号:2009SZ0227)

摘　　要：目的:建立葎草的薄层色谱鉴别方法。方法:以木犀草素对照品为对照,采用薄层色谱法进行鉴别研究。结果:供试品色谱中,在与对照品色谱相应的位置上,显相同颜色的荧光斑点,以甲苯-乙酸乙酯-甲酸(8∶2∶0.5)展开后Rf值适中,斑点清晰。结论:建立的薄层鉴别方法对葎草中木犀草素的鉴别有较好的适应性,重现性好,为完善其质量标准奠定了基础。Objective：To establish a Thin-Layer Chromatography（TLC） identification method for Humuli Scandens.Method：Using TLC with luteolin as the reference substance.Result：Both luteolin and Humuli Scandens had the same main spot and color on the plate.The Rf value was moderate and the spot was clear by using toluence-ethyl acetate-formic acid（8∶ 2∶ 0.5） as the developing solvent substances.Conclusion：The method is adaptable and reproducible to identify luteolin of Herba Humuli Scandens,which provide a basis for the quality control of Herba Humuli Scandens.

关 键 词：葎草 木犀草素 薄层色谱 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]