基于色彩色差计的川楝子炮制前后含量和颜色变化  

Comparison of Contents and Color Changes in Fructus Toosendan before and after being Processed

作　　者：艾莉[1] 陈君程[2] 张继良[1] 刘文刚[1] 吴纯洁[1] 孙灵根[1] 

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]西南民族大学,四川成都610041

出　　处：《成都中医药大学学报》2011年第4期81-83,90,共4页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：四川省科技支撑计划项目(编号:2008SZ0142)

摘　　要：目的:建立了川楝子药材中川楝素含量的高效液相方法,采用在线式非接触红外控温炒药机炒制川楝子,比较川楝子炒焦前后川楝素的含量变化及药材颜色变化。方法:采用色彩色差计测量炒焦前后药材颜色变化;采用色谱柱为Wondasil C18柱,甲醇-水(1∶1)为流动相,检测波长为217 nm,测定川楝素。结果:川楝素平均回收率为100.16%,RSD=1.57%(n=6);10批川楝子经过炮制后含量下降平均为37.69%(RSD=2.19%),川楝子焦品总色度值为41.97(RSD=为0.28%)。结论:用于测定川楝素的高效液相法精密度和重现性较好,该方法简单、方便、可靠,可作为川楝子的含量测定方法;采用在线式非接触红外控温炒药机炒制的10批川楝子,在炮制前后的含量变化以及炮制品的颜色方面稳定性较高。Objective： In this study,authors created a process to measure the contents of Toosendanin,an effective component in Fructus Toosendan,using HPLC.Use of on-line non-contact infrared temperature drug machine to stir-frying Fructus Toosendan,compare the toosendanin in Fructus Toosendan content and color changes before and after stir-frying.Methods：It was carried out on a Wondasil C18 column with a mobilephase methanol-water（11）,and a detection wavelength of 210nm for Toosendan.Results：The averaged recovery of Toosendanin reached 100.6%,RSD=1.57%（n=6）;average contents decreased 37.69%（RSD= 2.19%）of 10 batches Fructus Toosendan after stir-frying,Fructus Toosendan total color value of processed products 41.97（RSD= 0.28%）.Conclusion：Precision and reprod uction of the method is preferable.The method is simple,convenient,reliability and can be as a kind of method to determine the contents for Fructus Toosendan;Use of on-line non-contact infrared temperature drug machine to stir-frying 10 batches Fructus Toosendan,it has a higher stability of contents and color changes in Fructus Toosendan before and after being Processed.

关 键 词：川楝子 川楝素 在线式非接触红外控温炒药机 色彩色差计 HPLC 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]