骨髓间充质干细胞与聚乳酸一羟基乙酸支架材料相容性研究  

Compatibility of bone marrow mesenchymai stem cells and polylactic glycolic acid scaffold

作　　者：符厚圣[1] 周兴[1] 桂有富[1] 郑煜[1] 潘建刚[1] 

机构地区：[1]广州医学院第二附属医院泌尿外科,广州510260

出　　处：《中华实验外科杂志》2012年第2期286-288,共3页Chinese Journal of Experimental Surgery

基　　金：广东省自然科学基金资助项目（10151018201000019）

摘　　要：目的观察骨髓间充质干细胞（BMSCs）及其分化的神经样细胞与聚乳酸一羟基乙酸（PLGA）支架材料的相容性，并探讨两者复合修复周围神经损伤的效果。方法（1）在体外，通过荧光显微镜、流式细胞仪、扫描电镜等技术观察BMSCs在PLGA上的生长及分化成神经样细胞的情况；（2）在体内，取右侧坐骨神经损伤的sD大鼠，随即分为4组，分别移植单纯PLGA、BMSCs—PLGA、神经样细胞一PLGA及直接缝合神经，术后斜板试验观察4组动物下肢功能情况；60d后处死大鼠，取神经标本做病理切片。结果（1）在体外，BMSCs在PLGA上生长良好，与同期培养板里面的细胞比较，吸光度值（A）差异无统计学意义（P〉0．05）；（2）在体内，BMSCs及其神经样细胞复合PLGA后能修复损伤的坐骨神经，促进大鼠下肢运动功能的恢复。术后3周，各组大鼠斜板试验的角度分别是：直接缝合组（38．32±5．51）度、单纯PLGA组（40．27±2．74）度、BMSCs—PLGA组（45．14±3．43）度、神经样细胞．PLGA组（60．56±4．70）度，且神经样细胞．PLGA组显著高于其他组（P〈0．01）；病理切片显示支架与宿主神经组织有良好的生物相容性。结论BMSCs能诱导分化生成神经样细胞，而PLGA与两者有良好的生物相容性。Objective To study separately the compatibility between bone marrow mesenchymal stem cells （BMSCs） and polylactic glycolic acid （PLGA） scaffolds, or between differentiated neural-like cells and PLGA, and to explore the effects of both composites repairing peripheral nerve injury. Methods （ 1 ） In vitro, BMSCs were cultured by differential adherence, and composited PLGA, via fluorescence mi- croscope, flow cytometry（FCM） and scanning electron microscope was also applied to investigate the information of BMSCs＇ adhesion and induction into neural-like cells in the scaffolds. （2） In vivo, SD rat models with the injury for right sciatic nerve, were randomly divided into 4 groups. Bold scaffold, BMSCs-PLGA complex, and neural-like cells-PLGA complex were separately transplanted into SD rat models, and direct never suture. The ramp test was used to obsvered lower extremity motor function of SD rat models after oper ation. Nerve biopsy specimens was assayed after SD rat were killed in 60 days. Results （ 1 ） In vitro, BM- SCs can be exactly grow on the PLGA. It is have no different in optical density value（OD） compared to ceils inside the culture plates （ P 〉 0. 05 ）. （In vivo, BMSCs and nerve-like cells combined with PLGA, which can restore to peripheral nerve injury, and promote the recovery of lower extremity motor function of SD rat models. After operation for 3 weeks, the angle of ramp test in direct suture groups, bold scaffold groups, BM- SCs-PLGA groups, and neural-like cells-PLGA groups was （38. 32±5.51 ）o, （40. 27±2. 74 ）o, （45.14±3.43 ） o, （60. 56 ±4. 70） 0, and neural-like cells-PLGA groups was significantly higher than other groups. Pathologieal examination showed nerve tissue with the host stand a good biocompatibility. Conclusion BMSCs can be induction into neural-like eells;PLGA have good compatibility with them.

关 键 词：骨髓间充质干细胞 神经样细胞 支架材料 相容性 

分 类 号：R318.08[医药卫生—生物医学工程]