钛合金颗粒对小鼠成骨细胞增殖及核因子-κB受体活化因子配体的影响  

Effects of titanium alloy particles on cell proliferation and expression of receptor activator of nuclear factor-KB ligand gene in osteoblasts of mice

作　　者：刘林[1] 谢守祥[1] 赵宁军[1] 姚爱明[1] 秦宏敏[1] 许铁[2] 

机构地区：[1]徐州医学院附属医院急救中心,徐州221002 [2]徐州医学院救援医学研究所

出　　处：《中华实验外科杂志》2012年第2期305-307,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察钛合金颗粒对体外培养小鼠成骨细胞增殖以及核因子（NF）-κB受体活化因子配体（RANKL）的影响，探讨假体周围骨溶解机制。方法体外培养小鼠成骨细胞，随机分为空白对照组和钛合金颗粒处理A（0．01％）、B（0．10％）、C（1．00％）组。采用细胞计数试剂盒（CCK）-8法检测培养24、48、96、168h成骨细胞增殖活性。分别采用逆转录-聚合酶链反应（RT-PCR）、酶联免疫吸附试验（ELISA）检测培养24、48h后成骨细胞的RANKLmRNA表达及培养上清液的RANKL含量。结果培养48h，A组、B组与对照组比较细胞增殖活性差异无统计学意义（P〉0．05），C组与其他各组比较细胞增殖活性明显降低（P〈0．01）。培养168h，B组细胞增殖活性下降，和对照组比较差异有统计学意义（P〈0．01）。培养24、48h，和对照组比较，各实验组RANKLmRNA表达及分泌量均明显升高（P〈0．01），各实验组之间差异有统计学意义（P〈0．01）。结论钛合金颗粒可降低成骨细胞增殖活性，促进成骨细胞RANKL表达与分泌，是假体周围骨溶解机制之一。Objective To observe the effect of titanium alloy particles on the cell proliferation and the expression of gene receptor activator of nuclear factor-t^B （NF-KB） ligand （RANKL） in osteoblasts of mice, and to investigate the mechanism of periprosthetic osteolysis. Methods Osteoblasts were cultured in 10% serum medium containing different concentrations of titanium alloy particles. The cells were randomly divided into four groups. In the experimental groups A, B and C, osteoblasts were cultured with 0. 01%, 0. 10% and 1.00% concentrations of titanium alloy particles, respectively ; in the control group, no titani- um alloy particles were given. Cell Counting Kit-8 （ CCK-8 ） test was used to assess the cell proliferation at 24, 48, 96, 168 h, respectively. At 24 and 48 h, reverse transcription-polymerase chain reaction （RT- PCR） and enzyme linked immunosorbent assay （ELISA） were performed to detect the RANKL expression in osteoblasts and the intensity of the concentrations of RANKL in the supernatant of medium, respectively. Results The proliferation of osteoblasts had no differences between experimental groups A or B and control group at 48 h （ P 〉 0. 05 ）. The proliferation of osteoblasts in the experimental group C was significantly re- duced as compared with other groups at 48 h （P 〈 0. 01 ）. Meanwhile the titanium alloy particles up-regu- lated the mRNA and protein expression of RANKL （ P 〈 0. 01 ）. Conclusion The proliferation of osteo- blasts is decreased with exposure to titanium particles and different concentrations of titanium alloy particles could up-regulate the mRNA and protein expression of RANKL, which may be one of the meehamisms for the periprosthetic osteolysis.

关 键 词：钛合金颗粒 成骨细胞 核因子-KB 骨溶解 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]