腹主动脉粥样硬化斑块中纳米细菌的培养与鉴定  

The culture and detection of nanobacteria in abdominal aorta atherosclerotic plaque

作　　者：耿文茂[1] 张倩[2] 吴亚光[1] 秦成坤[1] 苏忠学[1] 

机构地区：[1]山东大学附属省立医院肝胆外一科,济南250021 [2]济南市长清区人民医院内三科

出　　处：《中华实验外科杂志》2012年第2期321-323,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察腹主动脉粥样硬化斑块中是否存在纳米细菌，并对其进行鉴定。方法12例腹主动脉粥样硬化斑块及12例正常动脉血管组织研磨，取上清液培养。8周后收集培养瓶底白色粉末，用纳米细菌单克隆抗体间接免疫荧光法对其进行检测，并利用电镜进一步观察。结果12例动脉粥样硬化斑块中10例纳米细菌检测阳性，2例阴性，12例正常动脉血管组织中1例纳米细菌检测阳性，11例阴性，差异有统计学意义（P〈0．05）。纳米细菌单克隆抗体间接免疫荧光检测时可发出绿色荧光，电镜下测量纳米细菌直径约为100～300nm。结论腹主动脉粥样硬化斑块中存在纳米细菌，它可能导致粥样硬化斑块不稳定。Objective To investigate whether the nanobacteria exists in abdominal aorta athero- sclerotic plaque or not and detect it. Methods 12 case of abdominal aorta atherosclerotic plaques and 12 case of normal artery were grinded, the supernatant fluid was cultured for eight weeks. Subsequently, the white extract from the culture flask was detected by nanobacteria monoclonal antibody immunofluorescence and electron microscope. Results Nanobacteria was positive in 10 case of atherosclerotic plaques but only in 1 case of normal artery, the difference was statistically significant （ P 〈 0. 05 ）. Green fluorescence could be present during indirect immunofluorescence detection. The size of nanobacreria was measured ranging from 100nm to 300nm by electron microscope analysis. Conclusion Nanobacteria was detected in abdomi- nal aorta atherosclerotic plaques and might led to atherosclerotic plaques instability.

关 键 词：纳米细菌 粥样硬化斑块 不稳定 免疫荧光 

分 类 号：R543.12[医药卫生—心血管疾病]