蚕豆种质资源清蛋白遗传多样性分析  

Albumin Genetic Diversity Analysis of Faba Bean(Vicia faba L.)Germplasms

作　　者：石建斌[1] 侯万伟[2] 刘玉皎[2] 马晓岗[2] 

机构地区：[1]青海大学,西宁810016 [2]青海省农林科学院,西宁810016

出　　处：《植物遗传资源学报》2012年第1期22-28,共7页Journal of Plant Genetic Resources

基　　金：国家"973"项目(2010CB134408);现代农业产业技术体系建设专项(CARS-09-G4);青海省高校"135高层次人才培养工程"项目

摘　　要：利用SDS-PAGE对101份蚕豆种质资源进行了清蛋白遗传多样性分析,共检测出蛋白带2625条。除共有带外,迁移率不同的谱带类型36种,其中相对分子量为92kD、75kD、62kD、40kD、34kD、17kD、13kD的谱带在检测的种质材料中出现的频率最高,分别为92.08%、90.10%、99.01%、95.05%、95.05%、98.02%、95.05%。其余29种谱带类型具有较强的多态性,多态性谱带平均为16.09条,多态性比例为44.69%。每份种质材料的清蛋白谱带数介于21～31条之间,平均25.99条;供试种质间遗传相似系数0.6111～0.9722,平均0.7122。3个地理类群内多样性指数0.9879,类群间多样性指数0.0121,表明蚕豆清蛋白的变异主要来源于类群内。聚类分析将参试种质分为6类,与以往蚕豆种质分类研究结果类似,表明清蛋白能在一定程度上反映种质间的亲缘关系。研究结果对于蚕豆蛋白质品质育种具有一定的参考价值。In order to investigate genetic diversity of albuminous loci,101 genotypes of faba bean（Vicia faba L.）were analyzed by SDS polyacrylamide gel electrophoresis（SDS-PAGE）.A total of 2625 protein bands were detected in tested genotypes.Except common bands,the protein bands of 92kD,75kD,62kD,40kD,34kD,17kD and 13kD presented the highest frequencies of 92.08%,90.10%,99.01%,95.05%,95.05%,98.02% and 95.05% respectively.The other 29 polymorphic protein bands showed higher polymorphism with 16.09 polymorphic bands in average.Each genotype had 21~31 albumin bands,with an average of 25.99.The genetic similarity of the tested genotypes varied from 0.6111 to 0.9722,with an average of 0.7122.The genetic diversity index within group was much higher than that among groups,which indicated the main variation of albumin occurred within populations.Cluster analysis divided the accessions into six major clusters,which somehow coincided with systematic classification of faba bean in previous studies of genetic resources.The research results provided reference to faba bean breeding on protein qualities.

关 键 词：蚕豆 清蛋白 SDS-聚丙烯酰胺凝胶电泳 遗传相似系数 聚类分析 

分 类 号：S643.6[农业科学—蔬菜学]