接触抑制和血清饥饿对细胞周期的影响  

Effect of contact inhibition and serum starvation on cell cycle

作　　者：任亮[1] 刘娣 付博[2] 房庆昌[1] 郭云云[1] 马红[2] 汪亮[2] 李忠秋[2] 李璐[3] 

机构地区：[1]东北农业大学动物科技学院,哈尔滨150030 [2]黑龙江省农业科学院,哈尔滨150086 [3]东北农业大学生命科学学院,哈尔滨150030

出　　处：《黑龙江畜牧兽医》2012年第2期5-7,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：转基因生物新品种培育项目(2008zx08006-003)

摘　　要：为了研究血清饥饿和接触抑制2种常用的处理方法对供核细胞周期的影响,试验采用血清饥饿和接触抑制2种方法处理经过纯化的供核细胞,并用流式细胞仪检测处理后的供核细胞的细胞周期,通过Modfit软件分析不同处理方法对细胞周期产生的具体影响。结果表明:血清饥饿和同接触抑制2种方法处理过的细胞停留在G0/G1期和S期的细胞百分数差异不显著,但是同对照组细胞相比差异显著(P<0.05);停留在G2/M期的细胞百分数差异显著(P<0.05),但是同对照组细胞相比差异不显著(P>0.05)。To research the effect of two commonly used methods on the cell cycle of donor cells, the experiment used the two methods of contact inhibition and serum starvation to treat the donor cells after purified, detecting the cell cycle by using flow cytometry. Modfit software was used to analyze the effect of different treatments on the cell cycle. The results showed that the percentage of cells between the two treat groups re- mained at the GO/G1 phase was not significant, but it was higher than that of the control group （ P 〈 0.05 ）. The percentage of cells between the two treat groups remained at the S phase was not significant, but it was lower than that of the control group （ P 〈 0.05 ）. The results demonstrate that contact inhibition and serum starvation have tremendous influence on cell cycle.

关 键 词：成纤维细胞 接触抑制 血清 饥饿 

分 类 号：Q24[生物学—细胞生物学]