周围神经损伤后脊髓神经元退变与组织型纤溶酶原激活物及其抑制物表达的关系研究  

Association between Neurodegeneration and Tissue Plasminogen Activator andPlasminogen Activator Inhibitor in Spinal Cord Following Peripheral Nerve Injury

作　　者：彭琳[1] 黄裔腾[2] 庄珊珊[1] 方裕森[1] 霍霞[2] 徐锡金[2] 

机构地区：[1]汕头大学医学院附属肿瘤医院检验科 [2]汕头大学医学院分析细胞学实验室,广东汕头515041

出　　处：《汕头大学医学院学报》2011年第4期214-218,F0003,共6页Journal of Shantou University Medical College

基　　金：广东省教育厅自然科学研究资助项目(Z03037)

摘　　要：目的：探讨周围神经损伤后脊髓神经元退变与组织型纤溶酶原激活物（tPA）及其抑制物1（PAI-1）、神经丝氨酸蛋白酶抑制剂（NSP）表达的关系。方法：雄性SD大鼠（56只）随机分为实验组（50只）和对照组（6只。无手术）。实验组行坐骨神经离断术（SCNT）,于术后1、4、7、14和21 d取伤侧脊髓L4~6节段,观察前角神经元形态学改变及tPA、PAI-1和NSP表达。结果：与对照组比,实验组L4~6tPA、NSP表达增加,NSP在1 d内达峰值后逐渐下调,tPA水平则在7 d升达最高峰,此时前角外侧核神经元存活率开始减少（P〈0.01）。术后21 d,tPA仍未恢复正常水平（P〈0.05）,脊髓前角运动神经元存活率61.6%。两组脊髓均未测到PAI-1表达。结论：SCNT后L4~6前角神经元退变,可能与损伤刺激脊髓灰质内神经元和小胶质细胞合成、释放tPA增加有关,同时,损伤也促使tPA抑制物NSP表达上调,后者可能发挥神经保护作用。Objective：To investigate the relationship between neurodegeneration and tissue plasminogen activator（tPA）and plasminogen activator inhibitors 1（PAI-1）and neuroserpin（NSP）following peripheral nerve injury. Methods：Fifty six male SD rats were randomly divided into normal group（6,unoperation）and experimental group（50,sciatic nerve transaction（SCNT））.The rats were removed with the lumbar from 4 to 6 of spinal cord after operation of 1,4,7,14,21 days.The morphology observation for neuron in spinal anterior horn was performed.The expressions of tPA,PAI-1 and NSP were determined.Results：Compared with the control group,the levels of tPA and NSP in L4～6 in the expermental group were upregulated.NSP and tPA peaked at first and 7th day respectively.From the time point of 7th day,the neuron loss was found（P〈0.05）.Until to 21th day,tPA had not decreased to a normal level（P〈0.05）,and the neuron survival rates was 61.6%.PAI-1 was detected in spinal cord between two groups.Conclusion：The upregulation of synthesis and release for tPA from neuron and microglia can account for motor neuron degeneration in homolateral spinal.NSP is stimulated and may play an neuroprotective role.

关 键 词：周围神经 坐骨神经 脊髓 神经元退变 组织型纤溶酶原激活物 

分 类 号：R338.21[医药卫生—人体生理学]