选择性抑制一氧化氮合酶对大鼠小体积肝移植的影响  

The role of selective inhibition of inducible nitric oxide synthase (iNOS) in small-for-size graft

作　　者：王平[1] 夏永祥[1] 张传永[1] 张峰[1] 王学浩[1] 

机构地区：[1]南京医科大学第一附属医院肝脏外科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2011年第12期1713-1716,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助(30772003)

摘　　要：目的:探讨诱导性一氧化氮合成酶(iNOS)在大鼠小体积肝移植模型中的作用。方法:选取清洁雄性Spraque-Dawley大鼠90只。采用改良的二袖套法建立吻合肝动脉的小移植物模型。实验分为3组:正常对照组、小体积肝移植组和氨基胍(AG)注射组。术后分别在1、3、6、12 h等4个时间点处死5只大鼠并采取部分肝组织及血清样本。检测指标:血清丙氨酸转氨酶(ALT),实时荧光定量RT-PCR测定肝组织iNOS mRNA的表达,免疫组化法测定肝组织iNOS蛋白的表达,HE染色光镜下病理观察等。结果:AG组与小体积肝移植组相比,实时荧光定量PCR显示:肝组织中iNOS mRNA表达量明显下降(P<0.05,n=5);免疫组化显示:肝组织中iNOS蛋白表达下降;但ALT明显升高(P<0.05,n=5);病理显示肝细胞大量坏死。结论:AG能抑制iNOS在大鼠小体积模型中的表达,但不能减轻肝脏损伤,反而加重了损伤。Objective：To study the role of iNOS in small-for-size graft. Methods： A non-arterialized partial liver transplantation model was used. Rats were divided into three groups：control group, small-for-size group, Aminoguanidine （AG） group. The expression of the iNOS mRNA and protein,the level of alanine aminotransferase （ALT） in serum,as well as histology were checked after reperfusion. Results： The expression of the iNOS in AG group was lower than that of the small-for-size group,but the expression of ALT in serum sample was significantly higher than that of the small-for-size group（P 〈 0.05,n = 5）. Histology showed necrosis was observed both in the small-for-size group and the AG group. Conclusion： AG can inhibit iNOS in small-for-size graft,but lead to the worse injury.

关 键 词：诱导性一氧化氮合成酶 小体积肝移植 氨基胍 

分 类 号：R657.3[医药卫生—外科学]