地塞米松和TLR_4在淡水淹溺型大鼠急性肺损伤中的作用  

The role of dexamethasone and TLR_4 in acute lung injury rats induced by freshwater drowning

作　　者：宋应健[1] 孟自力[1] 洪永青[1] 王立新[1] 高新怀[1] 

机构地区：[1]南京医科大学附属淮安第一医院呼吸科,江苏淮安223300

出　　处：《南京医科大学学报（自然科学版）》2011年第12期1777-1780,共4页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:探讨地塞米松和TLR4在大鼠淡水淹溺型肺损伤中的作用。方法:72只大鼠随机分为对照组、淹溺组和地塞米松组,通过淡水气道内吸入方法建立大鼠淡水淹溺型急性肺损伤模型,观察各时间点血气分析,检测0.5、2.0、4.0和8.0 h血清TNF-α、IL-6的含量以及肺组织湿/干重比值(W/D值)、MPO和TLR4 mRNA相对含量。结果:大鼠淡水淹溺型急性肺损伤模型建立成功;与对照组比较,淹溺组PaO2灌入淡水后急剧下降,随后逐渐回升,但仍维持较低水平。血清炎症因子TNF-α、IL-6分别在0.5、2.0 h开始表达,肺组织W/D值、MPO和TLR4 mRNA含量分别在淹溺后0.5、2.0和8.0 h升高。地塞米松可抑制炎症因子和TLR4 mRNA表达,减轻肺损伤。结论:TLR4参与淡水淹溺型大鼠肺损伤,地塞米松通过抑制其表达可减轻肺损伤。Objective：To observe the effects of dexamethasone and TLR4 in acute lung injury rats induced by freshwater drowning.Methods：Seventy-two rats were randomly divided into three groups：the control group,the freshwater drowning group and the dexamethasone group.A rat model of acute lung injury was induced by freshwater drowning by means of introduction freshwater into trachea of rats.At the time point of 0,0.5,2.0,4.0,8.0 and 24.0 h,the change of PaO2 was determined.At the time point of 0.5,2.0,8.0,and 24.0 h,the change of MPO,W / D and TLR4 mRNA in lung tissues,and the change of TNF-α and IL-6 in serum was determined.Results：Acute lung injury model induced by freshwater drowning was successfully reproduced in rats.Compared with the control group,PaO2 in the drowning group was decreased significantly at the time point of 0.5 h after infusion of freshwater,then elevated but maintained at a low level.The level of TNF-α and IL-6 in serum was increased at 0.5 and 2.0 h respectively.The value of W / D,MPO and TLR4 mRNA in lung tissues was enhanced at 0.5,2.0 and 8.0 h respectively.Dexamethasone alleviated lung injury induced by freshwater drowning through suppression of the expression of TLR4 mRNA and inflammatory factor.Conclusion：TLR4 may participate in acute lung injury induced by freshwater drowning,whereas,dexamethasone could suppress TLR4 mRNA and protect the lung from pulmonary freshwater drowning injury.

关 键 词：地塞米松 TOLL样受体4 淹溺 大鼠 急性肺损伤 

分 类 号：R563.5[医药卫生—呼吸系统]