高温(70℃)玉米秸秆水解液产氢行为及群落结构  

Study on Biological Hydrogen Generation and Microbial Community Structure Using Corn Stover Hydrolysate under High Temperature (70℃)

作　　者：张坤[1] 任南琪[1] 曹广丽[1] 王爱杰[1] 

机构地区：[1]哈尔滨工业大学城市水资源与水环境国家重点实验室,哈尔滨150090

出　　处：《环境保护科学》2011年第6期120-124,共5页Environmental Protection Science

基　　金：国家自然科学基金项目(哈尔滨产乙醇杆菌过量产氢代谢途径与机制解析30870037);国家创新研究群体基金(50821002)

摘　　要：考察了高温条件下(70℃)牛粪堆肥、土壤、厌氧污泥、腐烂秸秆种接种物利用玉米秸秆水解液的产氢行为。结果表明:牛粪堆肥接种时达到最大的产气量(1355.7mL/L)和氢气产量(608.4mL/L),随后依次为腐烂秸秆、厌氧污泥和土壤。修改的Gompertz方程可以较好描述产氢量随时间变化趋势(R2>0.99)。牛粪堆肥接种时达到最大的产氢潜力(676.0mL/L),而土壤接种时的迟滞时间最小(9.8h)。DGGE图谱显示:不同接种物对应不同的微生物群落结构。Bacillus thermozeamaize,Enterobacter sp.JDM-19和Thermoanaerobacterium polysaccharolyticum strain,KMTHCJT和可能分别是牛粪堆肥,厌氧污泥和腐烂秸秆接种条件下的关键产氢微生物。This study investigated the extreme thermophilic （70 ℃） hydrogen generation by using corn stover hydrolysate with four inoculums （cow dung compost, soil, anaerobic sludge, and rotten corn stover）. The experimental results indicated that the tests inoculated with cow dung compost presented the optimum biogas （1 355.7 mL/L） and hydrogen yields （608.4 mL/L）, and followed by rotten corn stover, anaerobic sludge, and soil. Modified Gompertz equation could well describe the time-course profile of hydrogen accumulation （R20.99）. The inoculation with cow dung compost reached the highest hydrogen generation potentiality （676.0 mL/L）, and the lag time was the lowest （9.8 h） under the inoculation with soil. DGGE profile showed that different inoculums corresponded to different microbial community structure. Bacillus thermozeamaize, Enterobacter sp. JDM-19, and Thermoanaerobacterium polysaccharolyticum strain KMTHCJT might be the key hydrogen generation microbes for cow dung compost, anaerobic sludge, and rotten corn stover respectively.

关 键 词：生物制氢 高温 接种物 玉米秸秆水解液 微生物群落结构 

分 类 号：X703.1[环境科学与工程—环境工程]