环介导等温扩增技术在呼吸道感染病原体检测中的应用  

Application of loop-mediated isothermal amplification for pathogens in respiratory tract

作　　者：陈愉生[1,2] 王大璇[1,2] 陈炆颖[1,2] 李鸿茹[1,2] 

机构地区：[1]福建医科大学省立临床医学院 [2]福建省立医院呼吸内科,福州350001

出　　处：《海峡预防医学杂志》2011年第6期1-2,17,共3页Strait Journal of Preventive Medicine

基　　金：福建省科技厅科技计划项目(No.2009Y0010);福建省卫生厅医学创新课题(No.2009CXB-2)

摘　　要：目的应用环介导等温扩增法(LAMP)对肺炎患者下呼吸道分泌物进行11种常见致病菌的核酸检测,探讨其临床应用价值。方法收集福建省立医院2009—2010年呼吸科诊断为下呼吸道感染患者的合格痰标本289例,行LAMP方法检测,与痰培养结果比较,分析其灵敏性和特异性。结果 LAMP检测时间仅需2～3h,明显短于痰培养;以细菌浓度>1×103为阳性阈值,敏感性74.0%,特异性95.3%,敏感性明显高于痰培养;其阳性率为42.9%(124/289)。结论 LAMP法可方便、快速检测肺部感染的常见病原菌,敏感性高于痰培养法。Objective To detect nucleic acids of 11 common pathogenic bacteria in lower respiratory tract from pneumonia patients by loop-mediated isothermal amplification（LAMP） and evaluate its clinical application.Methods Qualified sputum samples from 289 patients with lower respiratory infections were collected during 2009-2010 in Fujian provincial hospital and were detected by LAMP method.Comparing with routine sputum culture,the sensitivity and specificity of LAMP method were analyzed.Results It took 2~3 hours only for one LAMP assay,significantly shorter than that for sputum culture method.With1×103 copies of bacteria as a positive threshold,the sensitivity was 74.0% and specificity 95.3%.The sensitivity for LAMP was significantly higher than sputum culture.The positive rate was 42.9%（124/289）.Conclusion LAMP method is a convenient and rapid assay for detecting common pathogens of lower respiratory tract infections.The sensitivity of LAMP were higher than that of conventional culture method for clinical diagnosis.

关 键 词：病原检测 常见病原菌 环介导等温扩增方法 下呼吸道感染 临床应用 

分 类 号：R446.5[医药卫生—诊断学]