夏枯草迷迭香酸成分分析及其对α-糖苷酶活性影响研究  

Analyze on the Component of Rosmarinic Acid in Prunella vulgaris and Its Effect on α-Glycosidase Activity

作　　者：吴慧平[1] 王洪兰[2] 张喆[3,4] 许婷婷[1] 陈美娟[1] 张旭[1] 戴晓明[1] 

机构地区：[1]南京中医药大学基础医学院,江苏南京210046 [2]南京中医药大学药学院,江苏南京210046 [3]南京中医药大学 [4]江苏省肿瘤医院病理科,江苏南京210009

出　　处：《中药材》2011年第11期1712-1715,共4页Journal of Chinese Medicinal Materials

基　　金：国家科技支撑计划“硕导中医药诊疗技术及特色产品应用研究”重点项目(2006BAI11B08);中西医结合基础学科资助

摘　　要：目的:分析夏枯草中迷迭香酸成分及其对α-糖苷酶活性的影响。方法:用HPLC法对夏枯草提取物中迷迭香酸定性和定量分析。将迷迭香酸和拜唐苹,分别加入α-淀粉酶、α-葡萄糖苷酶、α-麦芽糖酶不同微量反应体系中,分别用Bernfeld法、pNPG法、GOD法测定其酶活性。结果:夏枯草提取物中存在迷迭香酸,其水提物和干粉,以及浸膏测得的迷迭香酸分别为0.1494、0.1657、0.2739 mg/g相当生药。迷迭香酸对α-糖苷酶活性均有抑制作用,对肠源型α-麦芽糖酶呈非竞争性抑制类型。结论:夏枯草水提物、浸膏对α-糖苷酶抑制作用与迷迭香酸有关。Objective：To analyze rosmarinic acid in Prunella vulgaris and its effect on the activity of α-gly cosidase.Methods： Q-ualitative and quantitative analyses of Rosmarinic acid in Prunella vulgaris were carried out by HPLC.The activity of different micro reaction systems like α-amylase,α-glucosidase and α-maltase,which were added to Rosmarinic acid and Acarbose,was determined by Bernfeld,pNPG and GOD.Results： The contents of Rosmarinic acid in the aqueous extract and its dry powder,and extractum of Prunella vulgaris were 0.1494,0.1657 and 0.2739 mg/g respectively,equal to crude drug.The Rosmarinic acid inhibited α-glycosidase,and its inhibition from α-maltase in small intestine was noncompetitive.Conclusion： The aqueous extract of Prunella vulgaris and its extractum′s inhibition from α-glycosidase is related to Rosmarinic acid.

关 键 词：迷迭香酸 Α-糖苷酶 酶抑制剂 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]