超临界二氧化碳萃取及水蒸气蒸馏法提取降香挥发油及其GC-MS分析  

Analyze on Chemical Compositions of Dalbergia odorifera Essential Oils Extracted by CO_2-Supercritical-Fluid-Extraction and Steam Distillation Extraction

作　　者：宋伟峰[1] 廖美金[2] 罗淑媛[1] 

机构地区：[1]中山大学附属第三医院,广东广州510630 [2]广东省中医院药学部,广东广州510120

出　　处：《中药材》2011年第11期1725-1727,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:用超临界二氧化碳萃取及水蒸气蒸馏法提取降香挥发油,并对挥发油进行GC-MS分析。方法:采用超临界二氧化碳萃取法(SFE-CO2)及水蒸气蒸馏法提取降香挥发油,并应用气相色谱-质谱联用(GC-MS)技术分析挥发油的化学成分,用峰面积归一法测定各化合物的相对含量。结果:在超临界CO2提取物中共鉴定出12种化合物,占总峰面积的34.49%,含量最高的是橙花叔醇(14.95%)、2-丙烯酸3-(4-甲氧基)乙酯(14.53%)、胜红蓟色烯(1.33%)。在水蒸气蒸馏法提取的降香挥发油中共鉴定出9种化合物,占总峰面积的30.62%,含量最高的是橙花叔醇(26.61%)、雪松醇(1.65%)。结论:超临界法较水蒸气法更加稳定可靠、重现性好,适用于降香挥发油的提取。Objective：To analyze the chemical compositions of Dalbergia odorifera essential oils extacted by CO2-supercritical-fluid-extraction（SFE-CO2）and steam distillation extraction（SD）.Methods：The essential oils of Dalbergia odorifera were extracted by steam distillation extraction and SFE-CO2.The chemical components were separated and analyzed by gas chromatography-mass spectrometry.Results：12 compounds were identified in SFE sample.The major components from essential oils were 2-propenoic acid-3（4-methoxyphenyl）-ethyl ester（14.53%）,nerolidol（14.95%）,ageratochromene（1.33%）.9 compounds were identified in SD sample.The major components from essential oils were nerolidol（26.61%）,cedrol（1.65%）.Conclusion：The SFE method is better than the SD method in reliability stability and reproducibility,and suitable for essential oils extraction of Dalbergia odorifera.

关 键 词：降香 挥发油 水蒸气蒸馏 超临界萃取 GC-MS 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]