木樨草素对脂多糖诱导的小胶质细胞炎性反应的抑制作用  

The Inhibitory Effect of Luteolin on Inflammation in LPS-induced Microglia

作　　者：黄丰[1] 朱苗苗[1] 邓华明[1] 张在军[2] 杨丽[3] 肖飞[4] 肖瑛[2] 聂红[1] 

机构地区：[1]暨南大学药学院中药药理教研室,广东广州510632 [2]暨南大学药学院新药研究所,广东广州510632 [3]暨南大学药学院中药学教研室,广东广州510632 [4]暨南大学医学院药理教研室,广东广州510632

出　　处：《中药材》2011年第11期1746-1749,共4页Journal of Chinese Medicinal Materials

基　　金：广东省医学科研基金(A2010344);暨南大学药学院211工程和重点实验室创新研究项目;国家自然科学基金(81073129)

摘　　要：目的:以脂多糖(LPS)刺激小胶质细胞BV-2,体外模拟神经退行性疾病的炎症模型,探讨木樨草素的抗炎作用和机制。方法:采用LPS(0.1μg/mL)刺激小胶质细胞建立炎症模型,MTT法检测木樨草素对BV-2细胞的毒性作用;硝酸还原酶法检测木樨草素对LPS刺激BV-2细胞一氧化氮(NO)表达量的影响;一氧化氮合酶分型法检测木樨草素对LPS刺激BV-2细胞一氧化氮合酶(iNOS)活性的影响;Western Blot法检测木樨草素对LPS刺激BV-2细胞TLR4蛋白表达影响。结果:木樨草素低、中、高剂量及阳性药物能抑制LPS诱导的BV-2炎性反应。结论:木樨草素能够抑制小胶质细胞活化产生的炎症反应,其抗炎作用的机制可能是通过抑制TLR4信号通路而发挥效用。Objective：To study the inhibitory effect of Luteolin on LPS-induced BV-2 cell.Methods：BV-2 cells were treated with LPS（0.1 μg/mL） for inflammation model;MTT assay was used to detect the viability of BV-2 cells;Nitric oxide（NO） was detected by the method of nitric acid reductase assay;Induce type II nitric oxide synthase（iNOS） enzyme activity was determined by type of nitric oxide synthase assay;TLR4 protein expression was examined by the Western Blot analysis.Results：Luteolin significantly decreased the NO production and TLR4 protein expression as well as iNOS activity in LPS-activated microglial cell.Conclusion：LPS induced activation of microglia lead to inflammatory response and its mechanism may be related to inhibiting TLR4 signaling pathway.

关 键 词：木樨草素 小胶质细胞 脂多糖 神经炎症 

分 类 号：R285.5[医药卫生—中药学]