5-羟甲基糠醛对过氧化氢诱导的凋亡海马神经细胞BCL-2、NF-κB表达的影响  

Effect of 5-Hydroxymethyl Furfural on BCL-2 and NF-κB Gene Expression of Apoptotic Rat Hippocampal Neurons Injured by H_2O_2

作　　者：顾海[1] 江亚兵[1] 姜海英[1] 许冬青[1] 俞婧婷[1] 丁霞[1] 赵凤鸣[1] 詹瑧[1] 王明艳[1] 

机构地区：[1]南京中医药大学,江苏南京210046

出　　处：《中药材》2011年第11期1753-1756,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(30772851);江苏省"六大人才高峰"第六批资助项目;江苏省研究生创新工程;南京中医药大学基础医学院青年课题;江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：目的:研究山茱萸活性成分5-羟甲基糠醛对H2O2诱导损伤的大鼠海马神经元凋亡及BCL-2、NF-κB蛋白表达的影响。方法:常规方法进行乳大鼠海马神经元的原代培养,复制体外H2O2氧化损伤模型,采用MTT法分析不同浓度5-羟甲基糠醛对大鼠海马神经元增殖的影响,流式细胞仪检测不同浓度5-羟甲基糠醛对大鼠海马神经元凋亡的影响,Western blotting检测BCL-2、NF-κB蛋白的表达。结果:MTT结果分析表明,模型组与对照组比较,差异显著(P<0.05),5-羟甲基糠醛高、中剂量组与模型组相比也有显著性差异(P<0.05);流式细胞仪检测结果显示,5-羟甲基糠醛高、中剂量组较模型组细胞凋亡率显著降低;Western blotting检测结果显示,5-羟甲基糠醛高、中、低剂量能提高BCL-2蛋白的表达,并对NF-κB的表达有抑制作用,并呈剂量依赖关系。结论:山茱萸活性成分5-羟甲基糠醛能抑制H2O2诱导的大鼠海马神经元的凋亡,其机制可能与其增加BCL-2蛋白表达,降低NF-κB蛋白表达有关。Objective：To investigate the effect of 5-hydroxymethyl furfural（5-HMF） on apoptosis and BCL-2,NF-κB gene expression of rat hippocampal neurons injured by hydroperoxide（H2O2）.Methods： Hippocampal neurons of newly born rat were cultured in vivo and injured by H2O2.Effect of different concentration of 5-HMF on cell viability was measured by MTT.Flow cytometer（FCM） was used to measure the apoptosis of rat hippocampal neurons pre-cultured with different concentration of 5-HMF,Western blotting was used to measure the expression of BCL-2 and NF-κB gene.Results：It revealed that the high and medium dosage of 5-HMF could increase the activity of rat hippocampal.The high,medium and low dosage of 5-HMF also increased the expression of BCL-2 gene and decreased the expression of NF-κB gene.Conclusion：5-HMF could restrain the apoptosis of cultured hippocampal neurons injured by H2O2.The mechanism may be related to increasing in BCL-2 level and decreasing in NF-κB level.

关 键 词：山茱萸 5-羟甲基糠醛 大鼠海马神经元 

分 类 号：R285.5[医药卫生—中药学]