响应面法优化超声提取柴胡皂苷a、c、d的工艺研究  

Optimization of the Ultrasound Assisted Extraction of Saikosaponins from Bupleurum falcatum Using Response Surface Methodology

作　　者：孙印石[1] 王建华[1] 朱玉霞[1] 将和凤[1] 张胜海[1] 

机构地区：[1]山东农业大学农学院/作物生物学国家重点实验室,山东泰安271018

出　　处：《中药材》2011年第11期1776-1780,共5页Journal of Chinese Medicinal Materials

基　　金：山东省农业良种工程资助项目([2008]167-2);山东省高等学校科技计划项目(J11LC24)

摘　　要：目的:应用响应面法优化3种柴胡皂苷a、c、d的最佳提取工艺。方法:采用超声提取法,在单因素试验基础上,根据中心组合实验设计原理采用3因素3水平的响应面分析法进行工艺优化,HPLC法测定柴胡皂苷a、c、d的提取率,色谱柱为symmetry C18柱(150 mm×4.6 mm,5μm),流动相为乙腈-水梯度洗脱,流速1.0 mL/min,检测波长200 nm,检测温度25℃。结果:柴胡皂苷a、c、d的最佳条件为:提取时间93 min、超声功率150 W、液固比60,3种总皂苷含量预测值为13.66 mg/g,验证值为13.64 mg/g,与预测值的相对误差为0.15%。结论:该工艺合理、可靠、有效成分提取率高。Objective：To investigate the optimization of extraction conditions of saikosaponins a,c and d from Bupleurum falcatum.Methods：Experimental factors and levels were firstly selected by one-factor test.According to the central composite experimental design principle,the response surface methodology with 3 factors and 3 levels was adopted.Used HPLC to determine the contents of saikosaponins a,c and d.Used column symmetry C18（150 mm×4.6 mm,5 μm）;Mobile phase was acetonitrile-water gradient elution,flow rate was 1.0 mL/min,the detection wavelength was 200 nm,detecting temperature was 25 ℃.Results：The optimum conditions of aikosaponins extraction were as follows：extraction time was 93 min,power was 150 W,solvent to solid ratio was 60.The predicted total saikosaponin extraction yield was 13.66 mg/g,while the actual extraction yield was 13.64 mg/g,with relative error of 0.15%.Conclusion：The optimum extraction process is reasonable,reliable and high yield of extracting effective content.

关 键 词：三岛柴胡 柴胡皂苷 超声波 响应面法 

分 类 号：R286.1[医药卫生—中药学]