附子双酯型生物碱的提取工艺研究  

Extraction Technology of Diester-type Alkaloids from Radix Aconiti Lateralis

作　　者：刘卓[1] 晏星[1] 徐春霞[1] 侯大斌[1] 袁小红[1] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621000

出　　处：《中药材》2011年第11期1781-1784,共4页Journal of Chinese Medicinal Materials

基　　金：四川省科技支撑项目(2008SZ0082)

摘　　要：目的:探讨附子中3种双酯型生物碱提取的影响因素,确定适宜的提取条件。方法:采用HPLC法测定中乌头碱、乌头碱和次乌头碱的含量,采用单因素试验法,对附子双酯型生物碱的提取工艺进行研究。结果:最佳双酯型生物碱提取工艺为:在15℃条件下,将附子粗粉置于10倍无水乙醇中冷浸3次,每次1 d,双酯型生物碱收率为0.57%,质量分数为18.81%,其中中乌头碱、乌头碱和次乌头碱收率分别为0.16%、0.032%和0.38%。结论:该提取方法高效、低毒、经济、操作方便,适合工业化生产。Objective：To determine the suitable extraction technology of diester-type alkaloids,such as mesaconitine,aconitine and hypaconitine from the roots of Aconitum carmichaeli（Radix Aconiti lateralis）.Methods：The contents of mesaconitine,aconitine and hypaconitine were determined by HPLC.Single-factor experiment was used to study the extraction factors.Results：The suitable extraction technology for diester-type alkaloids was as follows：coarse sizings of Radix Aconiti lateralis was extracted by 10 times ethanol for 3 times（each time for 1 day）at 15℃.The total yield of diester-type alkaloids was 0.57%,including 0.16% aconitine,0.032% mesaconitine and 0.38% hypaconitine,and the purity was 18.81%.Conclusion：The extraction technology is efficient,harmfulless,economical,convenient and can be used for industrial production.

关 键 词：附子 中乌头碱 乌头碱 次乌头碱 提取工艺 

分 类 号：R284.2[医药卫生—中药学]