2,4-D等植物生长调节剂对金银花叶片植株再生的影响  

Effects of Plant Growth Regulators on Plantlet Regeneration from Leaves of Lonicera japonica Thunb

作　　者：吴林[1] 刘奕清[2,3] 陈泽雄[2,3] 黄登艳[2,3] 

机构地区：[1]西南大学园艺园林学院,重庆400716 [2]重庆文理学院花卉研究所,重庆永川402160 [3]重庆市特色植物种苗工程技术研究中心,重庆永川402160

出　　处：《西南大学学报（自然科学版）》2011年第12期66-71,共6页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市森林工程科技支撑重大专项(CSTC;2009AB1122);重庆市科技创新能力建设项目(CSTC;2010CB1012);特色植物种苗工程重庆高校创新团队建设项目(201025);重庆文理学院校级科研项目(Y2010YL40)基金资助

摘　　要：以金银花叶片为外植体,研究了2,4-D等植物生长调节剂对愈伤组织诱导和植株再生的影响.结果表明:最佳愈伤组织诱导培养基为MB+1.0mg/L 2,4-D+0.5mg/L KT,愈伤组织诱导率达91.84%,生长良好;最佳不定芽分化培养基为MB+2.0mg/L 6-BA+0.1mg/L IBA,不定芽分化率达93.33%,且生长旺盛;最佳不定芽生根培养基为1/2MB+2.0mg/L IBA+0.2mg/L IAA,生根率达83.33%,且根较多,苗健壮.试管苗移栽成活率达89.3%.The leaves of Lonicera japonica Thunb were used as explants and the influences of 2,4-D and other plant growth regulators on callus induction and plantlet regeneration were studied.The results showed that the optimum media were MB＋1.0 mg/L 2,4-D＋0.5 mg/L KT for callus induction,with an induction rate of 91.84%;MB＋2.0 mg/L 6-BA＋0.1 mg/L IBA for adventitious bud differentiation,with a regeneration rate of 93.33%;and 1/2MB＋2.0 mg/L IBA＋0.2 mg/L IAA for plantlet rooting,with a rooting rate of 83.33%.Most（89.3%） of the plantlets survived after transplantation.

关 键 词：金银花 叶片 植株再生 

分 类 号：Q943.1[生物学—植物学]