孕早期甲氧滴滴涕染毒对孕鼠胎盘组织ERα基因表达的影响  

Effects of methoxychlor exposure on expression of ERα mRNA in mouse placenta during early pregnancy

作　　者：王晓蓉[1] 苑洪菲[1] 王黎娜[2] 于波 

机构地区：[1]武警黑龙江总队医院妇儿科,哈尔滨150076 [2]武警总医院妇产科,北京100039 [3]哈尔滨红十字医院,150070

出　　处：《武警医学》2012年第1期38-40,共3页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的探讨孕早期持久性暴露环境污染物甲氧滴滴涕(methoxychlor,MXC)对孕鼠胎盘组织雌激素受体α(es-trogen receptorα,ERα)表达的影响。方法孕鼠随机分组,每组10只,其中孕鼠皮下注射MXC为染毒组,皮下注射芝麻油为正常对照组,皮下注射雌二醇(estradiol,E2)为阳性对照组。采用半定量反转录-聚合酶链反应(RT-PCR)技术和免疫组化技术检测各组孕鼠胎盘组织中ERα的表达强度。结果孕鼠MXC染毒组ERαmRNA(0.68±0.11),与正常对照组(0.49±0.09)比较,差异有统计学意义(P<0.05);与阳性对照组(0.60±0.10)比较,差异无统计学意义(P>0.05)。免疫组化结果显示,MXC染毒孕鼠胎盘组织ERα阳性表达明显高于正常对照组。结论 ERα在MXC染毒孕鼠胎盘组织中的表达明显增高,可能是MXC影响胚胎发育的重要作用机制之一。Objective To investigate the effects of methoxychlor(MXC) exposure on the expression of estrogen receptor α(ERα) mRNA in mouse placenta during early pregnancy.Methods Pregnant mice were randomly divided into three groups with 10 for each,in which subcutaneous injection of MXC as the exposure group,subcutaneous injection of sesame oil as the normal control group,subcutaneous injection of estradiol(E2) as a positive control group.Placenta tissues were obtained from each group for analysis of ERα mRNA expression by using semi-quantitative reverse transcription polymerase chain reaction(RT-PCR).Results The expressions of ERα mRNA were significantly diferent between the MXC exposure group(0.68 ±0.11) and the positive control group(0.60±0.10)(P<0.05),but not diferent between MXC exposure group(0.68±0.11)and the normal control group(0.49±0.09)(P>0.05).Immunohistochemistry showed that the expression of ERα in pregnant mouse placenta exposed to MXC was significantly higher than that in the normal control group.Conclusions The expression of ERα in pregnant mouse placenta exposed to MXC is significantly increased,which may be one of the important mechanisms involved in the effects of MXC on embryonic development.

关 键 词：环境污染 甲氧滴滴涕 雌激素受体Α 

分 类 号：R715.3[医药卫生—妇产科学]