ISSR和SRAP标记技术在葫芦科植物种质资源研究中的应用  

Application of ISSR and SRAP Marker Technology on Germplasm Resource Research of Cucurbitaceous Plants

作　　者：蹇黎[1] 

机构地区：[1]贵州毕节学院地理与生命科学院,551700

出　　处：《长江蔬菜》2012年第2期13-16,共4页Journal of Changjiang Vegetables

基　　金：贵州省教育厅自然科学研究项目(2007082);国家自然科学基金项目(39770903)

摘　　要：阐述了简单重复序列间扩增(ISSR)和相关序列扩增多态性(SRAP)分子标记技术的基本原理和特点,综述与探讨了分子标记技术在葫芦科植物种质资源的亲缘关系鉴定与遗传多样性分析、分子遗传图谱的构建与目的性状基因的标记定位、基因库的构建和基因克隆、辅助育种选择及品种纯度鉴定等研究领域的广泛利用与推广,以期更合理高效地保护和利用葫芦科植物种质资源。The basic principles and characteristics of ISSR and SRAP molecular marker technology were summarized. And the application and extension states of the two molecular marker technologies were reviewed, such as on the relationship identification and genetic diversity analysis, construction of molecular genetic map and mapping of target genes, construction of gene pool and gene cloning, and molecular marker-assisted selection and identification of cultivar purity etc in germplasm resources of cucurbitaceous plants in this paper, expecting for more proper and efficient protection and application of the germplasm resources of cucurbitaceous plants.

关 键 词：ISSR SRAP 葫芦科植物 种质资源 应用 

分 类 号：S642[农业科学—蔬菜学]