疲劳型亚健康小鼠模型的研制  

Construction of fatigue-type sub-health mouse model

作　　者：刘艳艳[1] 程静茹[1] 余克强[1] 谭为[1] 欧阳明子[1] 陈淑娟[2] 黄建华[3] 罗仁[1] 赵晓山[1] 

机构地区：[1]南方医科大学南方医院中医科,广州510515 [2]广州军区广州总医院肾内科,广州510010 [3]广州军区广州总医院呼吸内科,广州510010

出　　处：《广东医学》2012年第1期21-24,共4页Guangdong Medical Journal

基　　金：广东省自然科学基金资助项目(编号:06105115);广州市科技计划项目(编号:2006Z3-E5051-3;2009Z1-E231)

摘　　要：目的研制疲劳型亚健康小鼠模型。方法疲劳型亚健康小鼠模型制备:强迫小鼠水中站立8h/d,分别观察连续造模5、9、12、16 d时小鼠行为学表现,以力竭游泳时间反映其疲劳程度,结合血浆生化、血常规及病理组织学检查等,确定造成疲劳型亚健康的最佳时间。疲劳型亚健康小鼠模型的评价:对上述创建的疲劳型亚健康小鼠模型进行评价,观察模型小鼠的疲劳持续时间。结果疲劳型亚健康小鼠模型制备:模型各组游泳时间与对照组比较显著下降(P=0.000)。与对照组小鼠比较,造模16 d组BUN、ALT、AST、TG改变明显(P<0.05);其余血生化、血常规及病理学检查未见明显异常。疲劳型亚健康小鼠模型的评价:模型小鼠力竭游泳时间明显下降;去除造模因素,休息恢复7 d后其力竭游泳时间有增长,与对照组比较差异有统计学意义(P<0.05);至恢复14 d后,小鼠力竭游泳时间与对照组比较差异无统计学意义(P>0.05)。结论采用强迫小鼠水中站立8h/d,连续9 d,成功制备疲劳型亚健康小鼠模型。该模型的稳定性、重复性较好,为疲劳型亚健康病理机制的研究及相关药物的筛选提供技术平台。Objective To establish and evaluate the fatigue-type sub-health mouse model. Methods Experiment 1： Mice in model groups were forced to stand in water 8 hours per day each for 5,9,12 and 16 days.The performances of mice behaviors,degree of fatigue,pathological signs in tissue,blood biochemistry results and other indicators were analyzed to determine the optimal time for this fatigue-type sub-health model.Experiment 2： Evaluation of the model was performed with the test of 7% weight-loaded forced swim.Results Experiment 1： In comparison with control group,the exhaustive swimming time was significantly shortened in all model groups（P=0.000）.Significant alterations of BUN,ALT,AST and TG were only observed in model 16d group（P0.05）,no significant change was revealed in the other model groups.Experiment 2： The exhaustive swimming time was significantly reduced in model mice.Although the fatigue degree abated after a rest of 7days free of modeling factors,swimming time was still significantly shorter than normal level（P0.05）.However,the swimming time became no significant difference compared with that of normal control mice with a recovery of 14 days（P0.05）. Conclusion Forcing mice to stand in water 8 hours per day for 9 days can successfully establish this mouse model of fatigue-type sub-health,with significant stability and reproducibility,providing foundation for further study of fatigue-type sub-health.

关 键 词：疲劳型亚健康 动物模型 小鼠 

分 类 号：R285.5[医药卫生—中药学]