双酚A对人类早孕绒毛组织β-HCG分泌功能的影响  

Influence of bisphenol A on morphology and β-HCG secretion of human villi during early pregnancy

作　　者：张毅[1] 朱洪[1] 吴丹梅[1] 谈顺[1] 黄元华[1] 

机构地区：[1]海南医学院附属医院生殖医学中心,海南海口570102

出　　处：《海南医学院学报》2012年第1期15-17,共3页Journal of Hainan Medical University

基　　金：海南省科技项目资助(061005)~~

摘　　要：目的:研究双酚A(BAP)水平与早孕人绒毛膜促性腺激素(β-HCG)分泌功能的关系,探讨BPA对绒毛和妊娠可能带来的影响。方法:以40例早期妊娠(6～8周)的新鲜绒毛组织进行体外培养,A、B、C组培养基中分别加入1 000×10-6、500×10-6、1×10-6g/L的BPA,D组为对照组,取第2、4天的培养液测定β-HCG。结果:随着培养时间的增加,含BPA的A、B、C组β-HCG水平呈递增变化,而D组呈递减变化(P<0.01)。以D组为基础,其他组与其差值为变化量,第2和第4天培养液中β-HCG增加与BPA呈正相关(P<0.05)。结论:BPA可以导致HCG分泌增加。Objective：To explore the correlations between bisphenol A（BPA） and the secretion function of the morphology and β-HCG of early human placental villi.Methods：Human villi collected from 40 cases of early pregnancy（6-8 weeks）were in vitro cultured as group A,B and C,and treated with BPA at 1000×10-6、500×10-6 、1×10-6 g/L separately.Group D was assigned as control group.Culture media were detected for β-HCG level on the 2nd and 4th day of culture.The cultured villi were used for pathological examination on 4th day.Results：As culture time prolonged,β-HCG levels of group A,B and C showed a gradual increase,and the increasing level was positively correlated with β-HCG level（P=0.002）.β-HCG level of group D showed reducing tendency,and was significant different from that of other groups（P0.01）.Conclusions： BPA can enhance β-HCG secretion of early villus.

关 键 词：双酚A 人绒毛膜促性腺激素(β-HCG) 体外培养 绒毛组织 

分 类 号：Q575.12[生物学—生物化学] R114[医药卫生—卫生毒理学]