葛根异黄酮对去卵巢大鼠骨骼质量的影响  

Effects of Total isoflavones of pueraria on bone quality in ovariectomized rats

作　　者：周艳[1] 扶晓明[2] 王维芬[3] 王友良[3] 

机构地区：[1]长沙医学院衡阳校区 [2]解放军169医院 [3]南华大学,湖南衡阳421001

出　　处：《微量元素与健康研究》2012年第1期1-3,7,共4页Studies of Trace Elements and Health

基　　金：湖南省教育厅科研基金资助项目(09C143)

摘　　要：目的:观察葛根异黄酮(TIP)对去卵巢大鼠骨骼质量的影响。方法:选用健康成年SD雌性大鼠40只,随机分为5组:假手术组、模型对照组、低、中、高剂量TIP组;假手术组切除卵巢旁脂肪组织,其余各组均切除双侧卵巢,建立去卵巢大鼠动物模型。按25、50、100 mg/kg 3个剂量的TIP分别给去卵巢大鼠灌胃3个月,实验结束后处死大鼠,用相关方法检测骨骼质量变化的指标。结果:中、高剂量TIP组动物的体重与相对应时相的模型组比较均有下降(P<0.05);中、高剂量TIP组大鼠股骨重量、骨钙含量与模型对照组比较增加(P<0.05);高剂量TIP组大鼠的血清钙含量增高,血清磷、骨钙素和碱性磷酸酶含量均低于模型对照组(P<0.05);中、高剂量TIP组大鼠的股中心BMD和远心BMD均随葛根异黄酮剂量的增加而增加,呈剂量—效应关系,与模型对照组比较差异均有显著性(P<0.05)。结论:一定剂量葛根异黄酮能提高骨钙含量、增加骨密度,对去卵巢大鼠骨骼质量有明显的改善作用。Objective： To investigate Effects of Total isoflavones of pueraria on bone quality in ovariectomized rats. Methods： Choose healthy adult female SD rats were randomly divided into five groups： sham operation group, model control group and Total isoflavones pueraria groups of low, medium,high - dose ; Sham group removal of Ovarian side adipose tissue , the ovariectomized were established rat model of other groups.. Press 25,50,100 mg / kg three doses of Total isoflavones of pueraria, give of ovariectomized rats were fed to three month; the experiment end rats were killed , detected with relevant indicators of bone quality changes. Results： Medium and high doses Total isoflavones group the animal weight and with relative phase model group were decreased （ P 〈 0. 05 ） ; Medium and high do- ses Total isoflavones group femur weight, bone calcium content compared and with the model control group increased （P 〈 0. 05） ; High - dose TIP rats increased serum calcium, serum phosphorus, osteocalcin, and alkaline phosphatase levels were lower than model group （P 〈 0. 05） ; Medium and high doses group of total isoflavones of pueraria rats distal femur BMD and central BMD with both doses of Pu- eraria lobata isoflavones increased in dose - response relationship with the model control group differences were significant （P 〈 0. 05）. Conclusion： A certain dose of Pueraria lobata isoflavones can increase bone calcium content, increased bone mineral density in ovariectomized rats significantly improved the quality of the role.

关 键 词：葛根异黄酮 卵巢切除术 骨质疏松 

分 类 号：R681[医药卫生—骨科学]