不同产地甘木通药材多谱图鉴定及质量控制  

Multiple Chromatograms Identification and Quality Control of Clematis filamentosa from Different Producing Areas

作　　者：陈道阳[1] 孙平川[2] 黄健玲[3] 邓亚利[3] 

机构地区：[1]广东省人民政府机关门诊部,广州510030 [2]陕西省中医药研究院,西安710003 [3]华南农业大学制药工程系,广州510642

出　　处：《中国药房》2012年第7期629-632,共4页China Pharmacy

摘　　要：目的:构建甘木通药材的薄层色谱(TLC)、高效液相色谱(HPLC)、傅里叶红外光谱(FTIR)、气相色谱-质谱(GC-MS)多谱图体系,控制其质量。方法:采用HPLC法测定药材中的山柰素含量,色谱柱为WatersXbridgeC18(250mm×4.6mm,5μm),流动相为乙腈-0.4%冰乙酸水溶液(梯度洗脱),检测波长为326nm;TLC法中甘木通石油醚部位与乙酸乙酯部位的展开剂分别为石油醚-甲醇(9:1)和氯仿-甲醇(9:1);采用FTIR法对石油醚和乙酸乙酯部位的特征吸收峰进行标注;采用GC-MS联用法分析甘木通的挥发油成分。结果:不同产地甘木通药材在TLC、HPLC与IR图中有共性的特征峰用于定性;HPLC测定山柰素含量可用于定量;GC-MS法共鉴定出37个组分。结论:多谱图体系可综合鉴定甘木通药材并用于其质量控制。OBJECTIVE：To establish the TLC,HPLC,FTIR and GC-MS fingerprints of Clematis filamentosa for quality control of it.METHODS：HPLC method was used to determine the content of kaempferide.The determination was performed on Waters Xbridge C18（250 mm×4.6 mm,5 μm） column with mobile phase consisted of acetonitrile-0.4% acetic acid（gradient elution）.The detection wavelength was set at 326 nm;Developer of ligroin-methanol（9：1） and chloroform-methanol（9：1）were used in ligroin extract and acetic ether extract respectively in TLC.Absorption peaks were labeled in petroleum ether and ethyl acetate by FTIR.The volatile oil components were analyzed by GC-MS.RESULTS：The common characteristic peaks in TLC,HPLC and IR fingerprints of C.filamentosa were used for qualitative analysis;the content of kaempferide was measured by HPLC;37 components were identified by GC-MS.CONCLUSION：Multiple chromatograms can be used for comprehensive identification and quality control of C.filamentosa.

关 键 词：甘木通 高效液相色谱法 山柰素 薄层色谱法 傅里叶红外光谱法 气相色谱-质谱联用法 多谱图体系 

分 类 号：R284.1[医药卫生—中药学] R927[医药卫生—中医学]