RP-HPLC法测定云香十五味丸中栀子苷的含量  

Content Determination of Gardenoside in Yunxiang Shiwu Pills by RP-HPLC

作　　者：陈晓北[1] 邹继红[1] 

机构地区：[1]赤峰学院医学院,内蒙古赤峰024000

出　　处：《中国药房》2012年第7期646-648,共3页China Pharmacy

摘　　要：目的:建立测定云香十五味丸中栀子苷含量的方法。方法:采用反相高效液相色谱法。色谱柱为Agilent Eclipse XDB-C18(150mm×4.6mm,5μm),流动相为水-乙腈(85:15),流速为1mL.min-1,检测波长为238nm。结果:栀子苷的进样量在0.784～3.920μg范围内与峰面积积分值呈良好线性关系(r=0.9998);平均加样回收率为99.3%,RSD=1.0%(n=9)。结论:本方法简便、快速、准确,适用于云香十五味丸中栀子苷的质量控制。OBJECTIVE：To establish the method for the content determination of gardenoside in Yunxiang shiwu pills.METHODS：RP-HPLC method was adopted.The separation was performed on Agilent Eclipse XDB-C18（150 mm×4.6 mm,5 μm） column with mobile phase consisted of water-acetonitrile（85：15） at the flow rate of 1 mL·min-1.The detection wavelength was set at 238 nm.RESULTS：The linear range was 0.784～3.920 μg（r=0.999 8） with an average recovery of 99.3%（RSD=1.0%,n=9）.CONCLUSION：The method is simple,quick,accurate,and is suitable for the quality control of gardenoside in Yunxiang shiwu pills.

关 键 词：反相高效液相色谱法 云香十五味丸 栀子苷 含量测定 

分 类 号：R283.64[医药卫生—中药学] R927.2[医药卫生—中医学]