电纺参数对PLGA/HA复合支架纤维形貌和直径的影响  

Effects of Electrospinning Parameters on Morphology and Diameter of Electrospun PLGA/HA Fibers

作　　者：张华林[1] 陈治清[2] 李咏梅[1] 冯佳[1] 

机构地区：[1]宁夏医科大学口腔医学院,宁夏银川750004 [2]四川大学华西口腔医学院,四川成都610041

出　　处：《哈尔滨医药》2011年第6期405-407,共3页Harbin Medical Journal

基　　金：教育部科学技术研究重点项目(211196);宁夏自然科学基金项目(NZ11124);宁夏医科大学特殊人才科研启动项目(XT201006)

摘　　要：目的通过静电纺丝的方法制备PLGA/HA复合支架,探讨电纺参数对复合支架纤维形貌和直径的影响。方法以三氯甲烷和N,N-二甲基甲酰胺为混合溶剂制备PLGA/HA纺丝液,通过调节PLGA的浓度、电压、接收距离,制备具有不同表面形貌的PLGA/HA复合纤维,采用SEM观察PLGA的浓度、电压、接收距离对纤维形貌和直径的影响。结果复合纤维的直径随PLGA浓度的增加而增加;随电压的增加而增加;随接收距离的增加先减小后增加。结论制备PLGA/HA复合支架较合适的电纺参数为:PLGA浓度25%,电压20KV,接收距离15cm。静电纺丝法制得的PLGA/HA复合支架有可能作为骨组织再生的支架在组织工程领域发挥作用。Objective To prepare electrospun PLGA/HA fibers by electrospun and investigate the effects of process parameters on the morphology and diameter of electrospun PLGA/HA fibers.Methods PLGA/HA solution was prepared with a mixture of TCM and DMF.The PLGA/HA fibers with different morphologies were fabricated via regulating PLGA concentration,voltage and tip-to-collector distance（TCD）.The morphology and diameter of the fibers were observed by SEM.Results The average diameters of the PLGA/HA fibers increased as the PLGA concentration increased;the average diameters of the fibers increased as the voltage increased;the average diameters of the fibers first decreased,then increased as the TCD increased.Conclusion Proper electrospinning parameters are 25% PLGA concentration,20 KV voltages and 15 cm TCDs.The electrospun PLGA/HA composite scaffolds could serve as an scaffold for bone tissue regeneration in tissue engineering.

关 键 词：PLGA HA 静电纺丝 电纺参数 

分 类 号：R319[医药卫生—基础医学]