Highly efficient modification of beta-lactoglobulin (BLG) gene via zinc-finger nucleases in cattle  

作　　者：ShengliYu  JunjieLuo  ZhiyuanSong  FangrongDing  YunpingDai NingLi 

机构地区：[1]State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China [2]Beijing GenProtein Biotechnology Co., Ltd., Room 306, Zhongding Building, Malianwa North Road No. 158, Haidian District, Beijing 100193, China

出　　处：《Cell Research》2011年第11期1638-1640,共3页细胞研究(英文版)

摘　　要：Dear Editor, Gene targeting is in widespread use as a gold stan- dard for determining the function of genes in mice and human embryonic stem cells [1]. However, the poor ef- ficiency of this technology has hindered its application to domestic animals,

关 键 词：基因打靶 Β-乳球蛋白 BLG 核酸酶 人类胚胎干细胞 修改 锌指 牛 

分 类 号：Q78[生物学—分子生物学] TS252.1[轻工技术与工程—农产品加工及贮藏工程]