河南省有偿供血人群HIV/HCV混合感染者HIV-1细胞嗜性的研究  

HIV-1 cell tropism of the population coinfected with HIV and HCV through paid blood donation in Henan province

作　　者：田地[1] 李林[2] 李天一[2] 刘红新[2] 李敬云[2] 徐小元[1] 

机构地区：[1]北京大学第一医院感染性疾病科,100034 [2]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室,北京100071

出　　处：《传染病信息》2011年第6期334-338,共5页Infectious Disease Information

基　　金：高等学校博士学科点专项科研基金(20090001110081);国家"十一五"科技重大专项(2008ZX10001-004;2008ZX10002-013)

摘　　要：目的探讨河南省有偿供血人群HIV/HCV混合感染者体内HIV-1细胞嗜性的分布情况。方法选取河南省经有偿供血途径感染HIV-1的161例感染者,采用酶联免疫法检测HCV抗体,并通过HCV核酸检测明确HIV/HCV混合感染。nested-PCR扩增env C2V3区,测序并分析HIV-1流行亚型,使用在线预测工具WebPSSM预测辅助受体的使用情况,分析V3环顶端四肽。结果河南省经有偿供血途径感染HIV-1人群的抗HCV阳性率为87.58%(141/161),HCV RNA阳性率为56.52%(91/161),HIV单独感染组和HIV/HCV混合感染组HIV-1均以B(B')亚型多见(96%和98.5%),V3环顶端四肽主要为GPGR(33.4%和33.8%)、GPGQ(22.0%和19.1%)和GPGK(14.0%和19.1%)。2组感染者的HIV-1均以使用CCR5辅助受体为主,HIV-1细胞嗜性在2组中的分布差异无统计学意义(P=0.971)。结论 HCV混合感染不影响HIV-1的细胞嗜性。HCV RNA检测有助于明确HIV/HCV混合感染者现症感染情况。Objective To investigate HIV-1 cell tropism of the population coinfected with HIV and HCV through paid blood donation in Henan province.Methods Totally 161 HIV-1 sero-positive blood samples were collected from individuals who were coinfected with HIV and HCV through paid blood donation in Henan province.HCV antibody was determined using enzyme-linked immunosorbent assay,and HIV/HCV coinfection was confirmed by the detection of HCV RNA.HIV-1 env C2V3 gene was amplified using nested-PCR and the subtype characterization was analyzed after sequencing.Co-receptor usage was predicted using biosoftware WebPSSM,and distribution of tetramer compositions on the tip of V3 loop was analyzed.Results Among the total 161 individuals infected with HIV-1 through paid blood donation,HCV sero-prevalence was 87.58%（141/161）,and the positive rate of HCV RNA was 56.52%（91/161）.The most predominant HIV-1 subtypes in both HIV monoinfection group and HIV/HCV coinfection group were B（B＇）（96% and 98.5%,respectively）,and the main tip motifs of V3 loop were GPGR（33.4% and 33.8%）,GPGQ（22.0% and 19.1%） and GPGK（14.0% and 19.1%）.Co-recepter CCR5 was mainly used in both HIV monoinfection group and HIV/HCV coinfection group,and the HIV-1 cell tropism between the two groups was not significantly different（P=0.971）.Conclusions The cell tropism of HIV-1 has no correlation with HCV infection.The decetion of HCV RNA helps to clarify the current infection situation in HIV/HCV coinfected population.

关 键 词：HIV-1 细胞 获得性免疫缺陷综合征 丙型肝炎病毒 感染 

分 类 号：R512.91[医药卫生—内科学]