高压氧联合亚低温对大鼠脑创伤细胞凋亡及Bcl-2表达的影响  

Effects of hyperbaric oxygen coupled with mild hypothermia on apoptosis and expression of Bci-2 following traumatic brain injury in rats

作　　者：祝子鹏[1] 秦军[1] 孙俊波[2] 汤敏[2] 吴建平[2] 闫玉华[2] 詹金凤[2] 李厚成[2] 

机构地区：[1]聊城市人民医院神经外科,山东省聊城252000 [2]湖北医药学院附属太和医院

出　　处：《中华航海医学与高气压医学杂志》2011年第6期347-350,共4页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的 探讨高压氧（HBO）联合亚低温（MHT）治疗对创伤性脑损伤（TBI）细胞凋亡及Bcl-2表达的影响.方法 制作大鼠自由落体脑挫伤动物模型,设立TBI常规治疗组（TBI组）、常规治疗＋ HBO组、常规治疗＋MHT组和常规治疗＋HBO＋ MHT组,应用原位末端标记法（TUNEL）和流式细胞术对各组大鼠脑挫伤病灶旁的细胞凋亡情况进行检测；使用免疫组化方法检测Bcl-2蛋白在脑组织中的表达情况.结果 常规治疗＋ HBO组（16.3±5.0）、常规治疗＋MHT组（18.7±6.9）和常规治疗＋HBO＋ MHT组（24.7±7.2） Bcl-2的表达均高于常规治疗组（8.9±4.2）（P＜0.05）,其中常规治疗＋HBO＋MHT组表达最高.结论 HBO联合亚低温治疗脑创伤可有效减少细胞凋亡及增加Bcl-2的表达.Objective To investigate the effects of hyperbaric oxygen （HBO） coupled with mild hypothermia （MHT） on apoptosis and expression of Bcl-2 following traumatic brain injury （TBI）.Methods The rat model of traumatic brain injury was developed with the free fall method,then,the animals were randomly assigned to one of the four groups： the routine therapy group,the routine therapy ＋ HBO group,the routine therapy ＋ MHT group,and the routine therapy ＋ HBO ＋ MHT group.Apoptosis of neuron cells adjacent to the traumatic cerebral injury foci was evaluated with TUNEL and flow cytometry,and the expression of Bcl-2 in the brain tissue was detected with immunohistochemistry.Results The expressions,of Bcl-2 in the routine therapy ＋ HBO（ 16.3 ±5.0）,the routine therapy ＋ MHT（ 18.7 ±6.9）,and the routine therapy ＋ HBO ＋ MHT groups（ 24.7 ± 7.2 ） were all higher than that of the pure routine therapy group （ 8.9 ± 4.2） （ P 〈 0.05 ）,with the expression of Bcl-2 in the routine therapy ＋ HBO ＋ MHT group being the highest.Conclusions HBO coupled with MHT could suppress the apoptosis and increase the expression of Bcl-2 efficiently.

关 键 词：高压氧 亚低温 细胞凋亡 创伤性脑损伤 Bcl-2 

分 类 号：R651.15[医药卫生—外科学]