TGF-β_1及FN在狼疮样小鼠肾组织中的表达及霉酚酸酯的干预作用  

Inhibition of Mycophenolate Mofetil on Over-expression of TGF-β_1 and FN in Renal Tissue of Murine Chronic Graft-versus-host Disease Lupus Nephritis

作　　者：曹永珍[1] 钱文娟[1] 王云[1] 许云[1] 卢国元[2] 

机构地区：[1]苏州大学附属张家港市第一人民医院肾内科,张家港215600 [2]苏州大学附属第一医院肾内科,苏州215006

出　　处：《中国中西医结合肾病杂志》2011年第12期1059-1062,I0011,共5页Chinese Journal of Integrated Traditional and Western Nephrology

摘　　要：目的:研究TGF-β1及FN在狼疮样小鼠肾组织中的表达及霉酚酸酯(MMF)的干预作用。方法:建立cGVHD狼疮样小鼠模型。按随机设计原则将模型动物分两组即模型组和MMF治疗组,另设正常对照组。检测各组小鼠血肌酐、尿素氮及尿蛋白的变化,用HE染色观察肾组织纤维化程度,以Real-time PCR检测各组小鼠肾组织中TGF-β1及FN的mRNA丰度,并用Western blotting方法检测各组小鼠肾组织中TGF-β1及FN的蛋白表达情况。结果:12周模型组的小鼠肾小球系膜节段、弥漫性增生,且血肌酐、尿素氮及尿蛋白较正常对照组均明显增加,Real-timePCR及Western blotting结果证实模型组小鼠的肾组织中TGF-β1及FN的mRNA和蛋白表达水平与正常组相比水平均明显增加,而MMF治疗组与模型组相比其TGF-β1及FN的mRNA和蛋白表达均降低,且血肌酐、尿素氮及尿蛋白水平也有所降低。结论:TGF-β1可能参与了狼疮肾炎的发病,MMF可能通过抑制肾组织TGF-β1的表达来改善狼疮肾炎的增生病变。Objective：To investigate the renal expression of the transforming growth factor-β1 （TGF-β1） and fibronectin（FN） in murine chronic graft-versus-host disease（cGVHD） lupus nephritis and to investigate the interventional effect of mycophenolate mofetilv（MMF） on it. Methods：Mice models of cGVHD lupus nephritis were established. Mice were randomly divided into three groups, namely control group, model group, MMF treated group. Histopathology, renal function and proteinuria were observed. The expressions of TGF-β1 and FN mRNA were assessed by Real-time PCR and the protein levels of TGF-β1 and FN were detected by Western blotting. Results：Real-time PCR and Western blotting results showed that the expressions of TGF-β1 and FN were significantly increased in renal tissue of cGVHD mice, and the level of BUN and Ccr in serum and proteinuria were also higher than those in control group. MMF ameliorated the histological lesions, maintained normal renal function, normalized proteinuria and suppressed TGF-β1 and FN expression. Conclusion：This study suggests that the TGF-β1 may be involved in the pathogenesis of lupus nephritis. The beneficial effect of MMF on lupus nephritis may be partially mediated by the inhibition of TGF-β1.

关 键 词：小鼠 狼疮肾炎 转化生长因子-β1 纤维连接蛋白 霉酚酸酯 

分 类 号：R593.241[医药卫生—内科学]