MECC分离并测定高粱及玉米中7种农药类环境激素  

Separation and Assay of 7 Endocrine Disrupting Pesticides in Corn and Durra by Micellar Electrokinetic Capillary Electrophoresis

作　　者：张玉芬[1] 齐景凯[2] 韩娜仁花[1] 于秀英[3] 

机构地区：[1]内蒙古民族大学分析测试中心,内蒙古通辽028043 [2]内蒙古民族大学生命科学学院,内蒙古通辽028043 [3]内蒙古民族大学农学院,内蒙古通辽028043

出　　处：《分析测试学报》2012年第1期40-44,共5页Journal of Instrumental Analysis

基　　金：内蒙古自然科学基金项目资助(2009MS0310)

摘　　要：用毛细管胶束电动色谱成功分离并测定了高粱和玉米中7种农药类环境激素(多菌灵、西玛津、莠去津、毒死蜱、溴氰菊酯、乙草胺和氯氰菊酯)的残留量。研究了电泳缓冲液及表面活性剂等因素的影响,在最佳分离条件(pH 9.0、20 mmol/L磷酸氢二钠+50 mmol/L SDS+5%乙腈为缓冲溶液,分离电压20 kV,检测波长222 nm,实验温度25℃,压力法进样,30 mbar×10 s)下,7种农药在28 min内得到基线分离,质量浓度与其峰面积在5.0～150μg.L-1范围内呈良好线性,检出限为0.6～3.0μg/L,回收率为97%～108%,相对标准偏差为2.2%～4.7%。该方法具有操作简单、快速方便及自动化程度高、重现性好等优点。A method was developed for the simultaneous separation and determination of carbendazim,simazine,atrazine,chlorpyrifos,decamethrim, acetochlor and cypermethrin in corn and durra by micellar electrokinetic capillary electrophoresis.The conditions for separation and detection of a mixture of seven pesticides were optimized.The results showed that,under the optimized conditions of running buffer：20 mmol/L Na2HPO4＋50 mmol/L SDS＋5% acetonitrile（pH 9.0）,separation voltage：20 kV,detection wavelength：222 nm,ambient temperature：25 ℃,pressure injection：30 mbar×10 s,the analytes were successfully separated within 28 min.The calibration curves of seven pesticides were linear in the range of 5.0-150 μg·L-1.The detection limits were in the range of 0.6-3.0 μg/L.This method was successfully applied in the analysis of seven pesticides in corn and durra samples with spiked recoveries of 97%-108% and RSD of 2.2%-4.7%.

关 键 词：胶束电动色谱 农药 分析 环境激素 

分 类 号：O657.8[理学—分析化学] F767.2[理学—化学]