非抑制电导检测离子色谱法测定硅藻培养液中硅酸根  

Determination of Silicate in Cyclotella Meneghiniana Kiits Solution Using Non-suppressed Conductance Detection Ion Chromatography

作　　者：李月[1] 刘晃[1] 

机构地区：[1]农业部渔业装备与工程技术重点实验室中国水产科学研究院渔业机械仪器研究所,上海200092

出　　处：《分析测试学报》2012年第1期66-70,共5页Journal of Instrumental Analysis

基　　金：国家科技支撑计划课题(2011BAD13B04);农业部"引进国际先进农业科学技术"自由申报项目(2010-S9)

摘　　要：采用戴安阴离子AS23(4 mm×250 mm)分析柱和AG23(4 mm×50 mm)保护柱、恒温电导检测器,建立了测定硅藻培养液中硅酸根(SiO32-)含量的非抑制电导检测离子色谱法。以4 mmol/L碳酸钠为淋洗液,淋洗液流速1.0 mL/min,进样体积100μL,采用峰高定量。该法测定SiO32-的线性范围为0～40 mg/L,检出限为0.017 mg/L,重复测定同一标样的相对标准偏差(RSD,n=7)小于5%,峰面积和峰高的RSD分别为10.9%、4.8%。测定不同生长时期硅藻培养液中的SiO23-,样品的加标回收率为102%～120%。该法灵敏、准确、简便易行,适用于硅藻培养液中SiO32-的检测。A novel method was developed for the determination of SiO2-3 in Cyclotella meneghiniana Kiits solution by non-suppressed conductance detection ion chromatography.The chromatography was performed on a Dionex anion AS23（4 mm×250 mm） analytical column and AG23（4 mm×50 mm） guard column with a constant temperature conductance detector.The experimental conditions were optimized,and the optimal chromatographic conditions were using 4 mmol/L sodium carbonate as eluent with a eluent speed of 1.0 mL/min,injection sample volume of 100 μL.The calibration curve of silicate was linear in the range of 0-40 mg/L.The detection limit was 0.017 mg/L.The relative standard deviation（RSD,n=7） for standard solution was lower than 5%.The RSDs for peak area and peak height measurements were 10.9% and 4.8%,respectively.The proposed method was applied in the determination of silicate in Cyclotella meneghiniana Kiits solution in different growing periods,and the spiked recoveries were in the range of 102%-120%.With the advantages of simplicity,good sensitivity,low cost,the method was suitable for the determination of silicate in cyclotella meneghiniana kiits solution.

关 键 词：离子色谱法 非抑制电导检测 硅酸根 硅藻 

分 类 号：O657.75[理学—分析化学] O741.4[理学—化学]