煅烧骨颗粒大小对兔颅骨缺损引导成骨作用的影响  

Effects of size of calcined bone particles on osteoconducing in rabbit with skull defect

作　　者：何钟勤[1] 高心[2] 田小华[3] 钟丞[2] 薛莹[2] 李倪娜[2] 王志刚 刘晓红[2] 孙晓宇[2] 

机构地区：[1]吉林大学中日联谊医院口腔科,吉林长春130033 [2]吉林大学口腔医院牙体牙髓科,吉林长春130021 [3]浙江省杭州市口腔医院儿童牙病科,浙江杭州310006 [4]中国石油吉化集团总医院口腔科,吉林吉林132000

出　　处：《吉林大学学报（医学版）》2012年第1期75-78,I0002,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研基金资助课题(200505146)

摘　　要：目的:观察煅烧骨颗粒大小对兔颅骨缺损引导成骨作用,阐明煅烧骨颗粒大小对成骨作用的影响。方法:将煅烧骨颗粒按直径大小分为4组,小颗粒组(0.1～0.3mm)、中颗粒组(0.3～0.6mm)、大颗粒组(0.6～0.9mm)和混合颗粒组(0.1～0.9mm),分别植于兔颅骨缺损中,于术后3、6、8和12周取材,采用改良的Gomori特殊染色,观察大体和组织学改变,并测定新骨形成积分光密度(A)值。结果:改良的Gomori染色病理切片,各组均见大量的毛细血管长入以及不同程度的新骨单位生长,小颗粒组和混合颗粒组在第12周均见骨小梁排列的成熟骨痂,各组A值均呈增加趋势。其中小颗粒组、混合颗粒组各时间点的A值均大于中颗粒组和大颗粒组(P<0.05);同时第3、6周小颗粒组A值高于混合颗粒组(P<0.05),第9、12周则低于混合颗粒组(P<0.05)。结论:不同大小颗粒煅烧骨均有成骨作用;小颗粒组和混合颗粒组成骨效果优于中等颗粒组,但混合颗粒组在修复后期表现为更强的成骨作用,提示煅烧骨颗粒大小可以作为筛选和构建仿生骨支架材料的重要参考指标之一。Objective To observe the effect of the size of calcined bone particles on osteoconduction and study its influence in osteogenesis.Methods According to the sizes of calcined bone particles they were divided into four groups：small particle group（group A,0.1-0.3 mm）,medium particle group（group B,0.3-0.6 mm）,large particle group（group C,0.6-0.9 mm） and mixed particle group（group D,0.1-0.9 mm）.Then they were rooted in the defective cranial of rabbit respectively.In the postoperative period of 3,6,8 and 12 weeks the materials were adoped to carry on improved Gomori special dyeing and general histologic observation,light density（A） values of new bone formation integral were determined.Results The results of the improved Gomori special dyeing showed that amount of capillary grew and new bone formation in different degrees was found.Both group A and group D diplayed mature callus formation.The A values of each group had an increasing tendency.The A values in group A and group D were significantly higher than those in group B and group C（P 0.05） at each time points.The A values in group A were significantly higher than those in group D（P 0.05） in 3 and 6 weeks and lower than those in group D in 9 and 12 weeks（P 0.05）.Conclusion The calcined bone particles with different sizes all play distinct roles in new bone formation.The effects on new bone fromation in group A and group D are stronger than those in group B and group D.But the effect in group D displays a stronger osteogenesis in the later period.It indicates that the size of calcined bone particles could be one of important indexes to screen and construct scaffold material in bone tissue engineering.

关 键 词：煅烧骨 成骨 颅骨缺损 

分 类 号：R336[医药卫生—人体生理学]