卵巢癌患者外周血CD4^+ CD25^(hi) CD127^(lo)调节性T细胞格局变化及临床意义  

Changed of CD4^+ CD25^(hi) CD127^(lo) regulatory T cells and its significance in patients with ovarian carcinoma

作　　者：荆结线[1] 乔丽娟[2] 郭爱芝[2] 杜丽莉[1] 田保国[1] 韩存芝[1] 赵先文[1] 

机构地区：[1]山西省肿瘤医院病因及标志物检测室,太原030013 [2]山西省肿瘤医院检验科,太原030013

出　　处：《中国免疫学杂志》2012年第1期49-53,共5页Chinese Journal of Immunology

摘　　要：目的:观察卵巢癌患者外周血中CD4+CD25hiCD127lo调节性T细胞格局变化及其相关免疫细胞因子TGF-β1、IL-10的变化及与临床病理特征之间的关系,探讨其临床意义。方法:采用流式细胞术(FCM)和酶联免疫吸附法(ELISA)检测70例卵巢癌患者、50例卵巢良性疾患及70例健康者外周血单个细胞中调节性T细胞的比率和血浆TGF-β1、IL-10的水平。结果:①卵巢癌患者外周血中CD4+CD25hiCD127loTreg调节性T细胞占CD4+细胞的比例为6.32%±1.46%(n=70),显著高于卵巢良性疾患4.03%±1.25%(n=50)和健康对照组3.21%±0.96%(n=70),均P<0.01。术后患者CD4+CD25hiCD127loTreg比率与术前比较无明显差异。②卵巢癌患者血浆中TGF-β1、IL-10水平(256.68±56.34)pg/ml、(28.24±3.12)ng/ml,明显高于良性疾患(156.48±43.68)pg/ml、(20.58±2.39)ng/ml与健康对照组(130.24±35.60)pg/ml、(18.38±2.98)ng/ml,有统计学差异,分别P<0.001,P<0.01。③术前卵巢癌患者外周血CD4+CD25hi CD127lo Treg比率、血浆中TGF-β1、IL-10水平与患者的临床分期、淋巴结转移以及远处转移有关,P<0.05～P<0.001。④相关分析显示,卵巢癌患者外周血CD4+CD25hiCD127lo Treg比率与血浆中TGF-β1水平、IL-10水平呈正相关,r=0.734,P<0.01;r=0.665,P<0.01。结论:①CD4+CD25hiCD127lo Treg在卵巢癌患者外周血中表达显著增高,这可能是卵巢癌患者免疫功能下降的一个重要原因,并与临床病理特征存在显著相关性;②卵巢癌患者血浆中抑制性细胞因子TGF-β1、IL-10水平明显升高,并与临床病理特征存在显著相关性;③CD4+CD25hiCD127loTreg与TGF-β1、IL-10水平存在正相关,CD4+CD25hiCD127loTreg可能通过产生抑制性细胞因子TGF-β1、IL-10对效应性T细胞发挥抑制作用。Objective：To investigate the proportion of the CD4＋ CD25hiCD127lo regulatory T cells and the levels of TGF-β1, IL-10 in the patients with ovarian carcinoma,and to study the correlation between CID4＋ CD25hiCD127lo regulatory T cells and clinical pathological characteristics of patients with ovarian carcinoma;To explore the correlation between CD4 ＋ CD25hiCD127lo Treg and TGF- β1, IL-10. Methods：The percentages of CD4 ＋ CD25hiCD127lo Treg in the peripheral blood lymphocytes and the levels of TGF-β1 ,IL-10 in plasma from 70 patients with ovarian carcinoma, 50 patients with benign diseases,70 healthy people were determined by FCM and ELISA. Results：①The patients with ovarian carcinoma,compared with benign diseases （4.03% ± 1.25% ） and the healthy people （3.21% ± 0.96% ）, had a significantly higher proportion （6.32 % ± 1.46% ） of CD4 ＋ CD25hi C D127lo Treg （P 〈 0.01 ） ; ②The levels of TGF-[31 （256.68 ± 56.34 pg/ml）, IL-IO （28.24 ± 3.12 ng/ml） were significantly higher than benign diseases [ （ 156.48 ± 43.68 ） pg/ml）, （20.58 ±2.39） ng/ml] and healthy people[ （ 130.24 ± 35.60） pg/ml, （ 18.38 ± 2.98） ng/ml] ,P 〈0.001-P 〈0, 01. ③the proportion of CD4 ＋ CD25hiCD127lo Treg,TGF-β1, IL-10 were closely related to FIGO,lymph node status ,distant metastases, （P 〈0.05 - P 〈 0.001. ④By nonparametrie correlation,The percentages of CD4＋C D25hiCD127lo Treg had positive correction with eoneentration of TGF-β1 （ r = 0. 734,P 〈 0.01 ）, IL-β1 （ r = 0. 665, P 〈 0. 01 ） . Conclusion： ①There was an increasing of the proportion of CID4 ＋CD25hi-CD127loTreg in the patients with ovarian carcinoma,which is probably an important reason of reduced immunological function of ovarian carcinoma, and There were closely correlation between C D4 ＋ CD25hi CD127loTreg and clinical pathological characteristics. ②the levers of TGF-β1, IL-10 was striking increase in the patients with ovarian carcinoma, and related to clinical pathological ch

关 键 词：卵巢癌 TGF-β1 IL-10 CD4+CD25hiCD127lo调节性T细胞 

分 类 号：R711.75[医药卫生—妇产科学]