犬源贾第虫EF1α基因的克隆及序列分析  

Cloning and Sequence Analysis of EF1α Gene of Giardia lamblia from Dogs

作　　者：李结[1] 王培园[1] 张萍[1] 刘远佳[1] 郭建超[1] 孟祥龙[1] 李国清[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《动物医学进展》2012年第1期53-56,共4页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(30972179)

摘　　要：以广州某宠物医院临床犬源贾第虫为研究对象,根据GenBank TM中登录的贾第虫EF1α基因序列(AF069575),设计1对引物EF/ER,采用PCR方法从贾第虫基因组DNA中扩增出目的片段,纯化后克隆至pGEM-T Easy载体,重组质粒通过菌液PCR鉴定后,对其进行序列测定及分析,旨在对广州犬源贾第虫进行分子鉴定。结果显示,该犬源贾第虫EF1α序列长为288bp,与预期目的片段一致,该序列构建的分子进化树表明该犬源贾第虫分离株为蓝氏贾第虫D型。说明EF1α适合做分子标记,可准确地对贾第虫进行基因分型,为贾第虫分子分类学及分子遗传学研究奠定了基础。In order to amplify the EF1α gene of Giardia lamblia isolated from dogs in Guangzhou,one pair of primers EF/ER were designed based on the published sequence of the EF1α gene in GenBankTM and the amplicons were cloned into pGEM-T easy vector.The recombinant plasmids were identified by PCR and the products were sequenced and analyzed.The results show that the EF1α of the dog-derived Giardia strain is 288 bp in length.The phylogenetic tree revealed that the dog-derived Giardia strain was Giardia lamblia Assemblage D.These results provided a foundation for further studies of molecular identification and molecular genetics of Giardia lamblia.

关 键 词：犬源贾第虫 EF1α基因 克隆 PCR技术 序列分析 

分 类 号：S852.722[农业科学—基础兽医学]