RNAi基因沉默的动力学研究进展  

Progress on RNAi Gene Silence Kinetics

作　　者：孟霞[1] 鲁秦安[2] 唐彩琰[1] 陈树林[1] 张文华[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]西安文理学院,陕西西安710065

出　　处：《动物医学进展》2012年第1期82-86,共5页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(30972151);"863"计划"十二五"农村领域国家科技计划项目(2011AA10A208)

摘　　要：RNA干扰(RNAi)是一种由小双链RNA介导的转录后基因沉默现象。在RNAi发挥基因沉默效能的过程中,许多因素都会直接或间接的影响最终的沉默效率和沉默时间。论文从siRNA类型的选择、siRNA的递送和细胞内的RNAi途径3个方面对RNAi途径中各个影响其效能发挥的因素及其解决方法做一综述,同时对描述RNAi基因沉默动力学的数学模型及其应用进行了介绍。RNA interference（RNAi） is a phenomenon of posttranscriptional gene silence mediated by small double-strand RNA.There are many factors affect the silence efficiency and time directly or indirectly when RNAi plays its role in silencie genes.This review discussed the main factors which affect RNAi effects and their solutions from three aspects which include the select of siRNA,the delivery of siRNA and the pathway of RNAi in cells.Besides,the mathematical models for describing RNAi gene silence kinetics and their applications were introduced briefly.

关 键 词：RNA干扰 短小干扰RNA 短发夹RNA 基因沉默 数学模型 

分 类 号：Q789[生物学—分子生物学]