猪肉质性状功能基因在野猪群体内的变异研究  

Research on Variation from Pork Quality Trait Function Gene in Wild Boar Population

作　　者：杨广礼[1] 郭艳[1] 闫永峰[1] 韩进诚[1] 付冬丽[1,2] 欧阳[1] 施传信[1] 徐珂[3] 

机构地区：[1]商丘师范学院生命科学学院,河南商丘476000 [2]甘肃农业大学动物科学技术学院,甘肃兰州730070 [3]河南师范大学生命科学院,河南新乡453700

出　　处：《中国畜牧兽医》2012年第1期136-141,共6页China Animal Husbandry & Veterinary Medicine

基　　金：河南省科技攻关项目(092102110088;2010B230007);商丘师范学院青年自然科学基金(2010QN19)

摘　　要：本试验旨在研究猪肉质性状功能基因突变位点在野猪群体里面的遗传变异规律。以MC4R基因(D298NG>A)、RYR1基因(c.1843C>T)、PRKAG3基因(1849G>A)和IGF2基因(3072G>A)突变位点为基础,通过PCR-RFLP、PCR-SSCP技术,对72头圈养野猪群体进行突变位点的多态性检测和群体遗传变异分析。结果表明,RYR1基因在c.1843C>T突变位点的CC基因型为野猪群体内的优势基因型,等位基因C是野猪群体内的优势基因,没有TT基因型,该突变位点处于不平衡状态(P<0.01和P<0.05)。在MC4R基因D298NG>A突变位点上,GG基因型为野猪群体内的优势基因型,等位基因G是野猪群体内的优势基因。而PRKAG3基因(1849G>A)的突变位点检测结果是GG基因型为野猪群体内的优势基因型,等位基因G是野猪群体内的优势基因,没有发现AA基因型。IGF2基因3072G>A在野猪群体内没有发现多态性,经测序验证全部为GG基因型。这3个基因突变都处于Hardy-Weinberg平衡状态(P>0.05)。本研究得出该野猪种群体遗传多样性和变异较小,符合其品种特性。The aim of this research was to understand the wild pig population genetic variation in different meat quality traits function genes. In this study, we chose MC4R gene （D298N G〉A）, RYR1 gene （c. 1843 C〉T） ,PRKAG3 gene （1849 G〉 A） and IGF2 gene（3072 G〉 A） mutation sites and used for genotyping by PCR-RFLP and PCR-SSCP methods using 72 DNA from wild boar experimental animals. The results showed that in the RYR1 c. 1843 C〉T mutation site, CC genotype was the wild hoar population dominant genotype, allele C was dominant gene within the wild boar population, no found ＂IT genotype and RYR1 c. 1843 C^T mutation site was not Hardy-Weinberg equilibrium（P^0.01 and P^0. 05）. The MC4R gene D298N G^A point mutation, GG genotype was the wild boar population dominant genotype, allele G was dominant gene within the wild boar population. With PCR- BsrB I-RFLP used for detection wild boar population, GG genotype was dominant genotype,allele G was dominant gene, no found AA genotype. No variation was detected in wild boar populations for IGF2 gene 3072 G〉A mutation. GG genotype was dominant geno- type in wild boar population by sequencing validated. Furthermore, the population genetic analytic results showed that these mutation sites were Hardy-Weinberg equilibrium（P〉0.05）. It was concluded that the wild boar population had lower genetic variation and diversity and consistent with the breed characteristic.

关 键 词：野猪 肉质性状 多态性 

分 类 号：S813.1[农业科学—畜牧学]