猪繁殖与呼吸综合征的ELISA抗体检测结果分析与应用  

Analysis and Application of ELISA Results for the Detecting Porcine Reproductive and Respiratory Syndrome Antibody

作　　者：李剑秋[1] 赵灵燕[2] 周彩琴[2] 吴赟竑 吴雪军[2] 倪柏锋[2] 徐辉[2] 

机构地区：[1]浙江工商大学杭州商学院,浙江杭州310035 [2]浙江省动物疫病预防控制中心,浙江杭州310020

出　　处：《中国畜牧兽医》2012年第1期178-180,共3页China Animal Husbandry & Veterinary Medicine

基　　金：浙江省重大科技专项农业项目(2007C12045)

摘　　要：本研究应用ELISA方法共检测25个猪场26个猪群507份血清的PRRS抗体水平,应用统计学方法分析各猪群的检测数据,结合猪群临床表现和病原污染情况的流行病学调查结果,可将猪群分为无PRRSV污染,临床健康;PRRS病毒污染,临床稳定;PRRS病毒污染,临床发病且控制困难等3种流行模式,并提出了应用猪群PRRS ELISA检测值标准差、个体检测极值及盒须图(Box-and-Whisker Plot)进行分类的具体指标和PRRS分类控制的建议。507 sera from 25 pig farms and 26 flocks were collected and detected the antibody level by ELISA.The results from each flock were analyzed by the statistics methods.Three PRRS modes of pig flocks could be divided by the antibody distribution,clinical sign and pathogen contamination：①clinical health,no PRRSV contamination;②clinical stable,PRRSV contamination;③clinical unstable,PRRSV contamination,without effective control measures.It was suggested that the indices of standard deviation,the greatest limited value and Box-and-Whisker Plot could be used for three modes＇ identification.The control measures for different mode were suggested.

关 键 词：PRRSV 抗体检测 流行模式 数据分析 

分 类 号：S855.3[农业科学—临床兽医学]