流式细胞术检测细胞亚群存活率的应用研究  

Detection on survival rate of different cell subpopulations in mixed cell population by flow cytometry

作　　者：刘阳[1] 赖翼[1] 李敏惠[1] 杨淑霞[1] 郑静[1] 阳泰[1] 李丽梅[1] 邓勇[1] 张强[1] 任荣梅[1] 李钱梅[1] 张帅[1] 李华[2] 邹强[1] 

机构地区：[1]成都医学院科研中心,610083 [2]成都军区总医院肿瘤科,610083

出　　处：《国际检验医学杂志》2012年第2期130-131,共2页International Journal of Laboratory Medicine

基　　金：教育部科学技术研究重点资助项目(209104);教育部新世纪优秀人才支持计划资助项目(NCET-09-0888);四川省青年科技基金资助项目(2010A08-443);成都医学院科研基金资助项目(CYZ09-019)

摘　　要：目的采用流式细胞术直接检测小鼠脾细胞中T细胞与Treg细胞存活率,探讨流式细胞术测定混合细胞中细胞亚群存活率的可行性。方法荧光抗体及碘化丙锭联合使用,测定小鼠脾细胞中T细胞与调节性T细胞的存活率。结果使用流式细胞术测定脾细胞中T细胞存活率时,r2=0.9925,斜率为1.012;测定调节性T细胞存活率时,r2=0.9831,斜率为0.9823。结论流式细胞术可以应用于测定混合细胞中细胞亚群的存活率,为药物筛选提供新的选择。Objective To detect cell survival rate of T cell and Treg cell in spleen cells by flow cytometry for finding out whether flow cytometry could be used for the detection of cell subpopulation survival rate in mixed cell population.Methods Flow cytometry was used to detect cell survival rate of T cell and Treg cell in spleen cells of mouse with fluorescent antibody and propidium iodide.Results For the detection on cell survival rate of T cell,the r2 and slope of flow cytometry were 0.992 5 and 1.012 respectively,and for Treg cell,were 0.983 1 and 0.982 3 respectively.Conclusion Flow cytometry could be utilized for detecting cell survival rate of subpopulation in mixed cell population and provide another choice of drug screening.

关 键 词：流式细胞术 存活率 细胞 细胞亚群 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]