两种不同溶血免洗方法对流式T淋巴细胞亚群检测的影响  

Effects of two different haemolyzing/no washing methods on T-lymphocyte subgroups detection by flow cytometry

作　　者：陈潇[1] 吴丽娟[1] 

机构地区：[1]成都军区总医院检验科,610083

出　　处：《国际检验医学杂志》2012年第2期151-153,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨甲酸溶血免洗法和NH4Cl溶血免洗法对流式T淋巴细胞亚群检测的影响程度及优劣,为流式T淋巴细胞亚群检测的标准化提供科学依据。方法采用三色流式细胞术FSC/SSC设门策略分析健康体检人群外周血T淋巴细胞亚群,调查甲酸溶血免洗法和氯化铵溶血免洗法及其溶血处理后上机间隔时间(IT)对检验结果的影响。结果甲酸溶血免洗法检测结果较稳定,室温避光放置2d或冰箱冷藏室放置3d检测结果无明显改变;NH4Cl溶血免洗法溶血后需尽快测定,室温避光放置或冰箱冷藏室放置6h检测结果明显降低。结论甲酸溶血免洗法更适合流式T淋巴细胞亚群的检测,其检测结果较NH4Cl溶血免洗法稳定性好。Objective To investigate the effects of methanoic acid haemolyzing/no washing method and ammonium chloride haemolyzing/no washing method on peripheral blood T-lymphocyte subgroups detection by flow cytometry for providing scientific data to standardize the detecting procedure.Methods The contents of peripheral blood T-lymphocyte subgroups were detected by a 3-color flow cytometry with FSC/SSC gating for lymphocytes in persons accepting health-examination.The samples were detected by flow cytometry using the two haemolyzing/no washing methods,respectively,at different interval time（IT） after haemolyzing and before detecting.Results The detection results of samples,treated by methanoic acid haemolyzing/no washing method could be stable for 2 days,when being stored at room temperature and protected from light,and for 3 days,when being stored in cold closet of refrigerator.Samples,treated by ammonium chloride haemolyzing/no washing method must be detected as soon as possible after being haemolyzed and the results decreased in 6 hours,whether samples were stored at room temperature and stored protected from light or stored in cold closet of refrigerator.Conclusion The methanoic acid haemolyzing/no washing method could be more suitable for detecting T-lymphocyte subgroups by flow cytometry than the ammonium chloride haemolyzing/no washing method.The results of methanoic acid haemolyzing/no washing method could be more stable.

关 键 词：流式细胞术 T淋巴细胞亚群 甲酸类 氯化铵 标本预处理 

分 类 号：R446.6[医药卫生—诊断学]