高效液相色谱法测定蔬菜中邻苯二甲酸二丁酯残留  

Residue Determination of Dibutyl Phthalate in Vegetables by HPLC

作　　者：王少杰[1,2] 孟雨吟[3] 李秋顺[1,4] 孙士青[1,2] 许强[5] 孟令玗[5] 

机构地区：[1]山东省生物传感器重点实验室,山东济南250014 [2]山东省科学院生物研究所,山东济南250014 [3]山东农业大学,山东泰安271018 [4]山东省科学院中日友好生物技术研究中心,山东济南250014 [5]山东省医学科学院,山东济南250062

出　　处：《山东农业科学》2012年第1期112-114,共3页Shandong Agricultural Sciences

基　　金：山东省科学院科技发展基金项目[科技合字(2010)第4号]

摘　　要：用高效液相色谱法测定了蔬菜中的邻苯二甲酸二丁酯残留含量。实验色谱条件为色谱柱:ZOR-BAX SB-C18 4.6 mm×150 mm,5μm;温度:室温(25℃);检测器:紫外检测器;检测波长:224 nm;进样量:10μl;流速:1.0 ml/min。检测方法在2～50μg/ml呈线性,最低检出限(3/n)0.025μg/ml,相关系数0.9991。蔬菜中邻苯二甲酸二丁酯含量0.45～7.70 mg/kg。实验表明邻苯二甲酸二丁酯在所检测的蔬菜中均有残留,可能与种植土壤污染有关。The residual content of dibutyl phthalate（DBP） in vegetables was determined by high performance liquid chromatography method（HPLC）.The experimental chromatographic conditions were as follows.The chromatographic column was ZORBAX SB-C18 4.6 mm × 150 mm,5 μm;the operational temperature was 25℃;the injection volume was 10 μl and the flow rate was 1.0 ml/min;the UV detector was used and the detecting wavelength was 224 nm.It was linear in the range of 2~50 μg/ml（r=0.9991,n=3）.The detection limit was 0.025 μg/ml and the DBP content in vegetables was 0.45~7.70 mg/kg.The results showed that DBP remained in all of the detected vegetables,which might be associated with the pollution of soil.

关 键 词：蔬菜 塑化剂 高效液相色谱 邻苯二甲酸酯 

分 类 号：O657.72[理学—分析化学] X503.231[理学—化学]