糖尿病微血管病变与血清同型半胱氨酸、氧化应激反应的相关性  

Relationship between serum homocysteine and oxidative stress in patients with Diabetic Microangiopathy

作　　者：魏剑芬[1] 王颖[1] 吴乃君[1] 陈冬[1] 马绍杰[1] 金秀平[1] 

机构地区：[1]河北联合大学附属医院内分泌科,唐山063000

出　　处：《中国综合临床》2012年第2期176-179,共4页Clinical Medicine of China

摘　　要：目的探讨糖尿病微血管病变患者血清同型半胱氨酸与氧化应激反应的相关性。方法测定80名健康人（对照组），100例无微血管病变2型糖尿病患者（DM组）、100例合并糖尿病肾病2型糖尿病患者（DN组）及100例合并DR2型糖尿病患者（DR组）血清同型半胱氨酸（Hcy）、丙二醛（MDA）、超氧化物歧化酶（SOD）及还原型谷胱甘肽（GSH）浓度。结果DM组、DN组、DR组Hcy浓度分别为（98．86±21．46）、（198．95±19．35）、（138．65±15．25）ng／L，MDA浓度分别为（17．49±1．64）、（28．89±2．14）、（22．47±1．86）nmol／L，均明显高于对照组（62．48±15．36）ng／L，（11．86±0．48）nmoL／L）（F值分别为7．95、6．89，P均〈0．01）；DN组及DR组均较DM组明显升高（P均〈0．01），DM组、DN组、DR组血清SOD浓度分别为（107．80±15．62）、（79．86±14．63）、（89．34±12．75）mg／L，GSH浓度分别为（179．26±25．81）、（143．36±21．75）、（156．96±19．35）mg／L，均低于正常对照组（128．32±19．21）mg／L，（237．38±27．31）mg／L（F值分别为7．89、8．76，P均〈0．01）；DN组及DR组均较DM组低（P均〈0．01）；同时DN组又低于DR组（P〈0．01）；血清Hcy与MDA浓度呈正相关（r=0．79，P〈0．05），与SOD、GSH浓度均呈负相关（r值分别为-0．71、-0．78，P均〈0．05）。结论糖尿病微血管病变患者血清同型半胱氨酸浓度升高，氧化应激反应增强，氧化应激与血清同型半胱氨酸浓度升高有关，血清同型半胱氨酸浓度升高，氧化应激促进糖尿病微血管病变发生发展。Objective To investigate the changes of serum homocysteine and it s relationship with oxidative stress and diabetic microangiopathy. Methods Eighty health participants were recruited as control 100 type 2 diabetis patients without diabetic microangiopathy were recruited as DM group, 100 type 2 diabetis patients with diabetic nephropathy were recruited as DN group, and 100 type 2 diabetis patients with diabetic retinopathy were recruited as DR group. Their serum levels of homocysteine, malonaldehyde （MDA）, superoxide dismutase （SOD） and glntathione （GSH） were measured. Results The homocysteine was （98.86 ± 21.46）, （ 198.95 ± 19. 35 ）, （ 138.65 ± 15.25 ） ng/L in the DM, DN and DR group respectively, which were signigicantly higher than that of （62.48 ±15.36） ng/L in the control group（ F = 7.95, P 〈 0. 01 ）. MDA was （ 17.49 ± 1.64）, （22.47 ± 1.86） and （ 22.47 ± 1.86 ） mmol/L, which was significantly higher than that of （ 11.86 ± 0.48 ） mmol/L in the control group（ F = 6. 89 ,P 〈 0. 01 ）. The homocysteine and MDA in the DN and DR group were both significantly higher than those in the DM group（P 〈0. 01 ）. The SOD and GSH was （ 107. 80 ± 15.62） mg/L and （ 179.26 ± 25.8 ） mg/L in the DM group, （79. 86 ± 14. 63 ） mg/L and （ 143.36 ± 21.75 ） mg/L in the DN group, （89. 34 ± 12. 75 ） mg/L and （ 156. 96 ± 19.35 ） mg/L in the DR group, which were significantly higher than those of （ 128.32 ± 19.21 ） mg/L and （237. 38± 27.31 ） mg/L in the control group （ F = 7.89 and 8.76 respectively, P 〈0. 01 ）. The SOD and GSH in the DN and DR group were both significantly lower than those in the DM group （ P 〈 0.01 ）, and the DN group was significantly lower than the DR group （ P 〈 0.01 ） . Serum homocysteine was positively correlated with MDA （ r = 0. 79, P 〈 0. 05 ）, and negtively correlated with SOD and GSH （ r = - 0. 71 and - 0. 78,P 〈 0. 01 ）. Conclusion Diabetic microangiopathy patients have high

关 键 词：2型糖尿病 糖尿病微血管病变 同型半胱氨酸 氧化应激 

分 类 号：R587.2[医药卫生—内分泌]