一氧化氮合酶/一氧化氮介导人参皂苷Rb1对心肌细胞肥大的抑制效应  

Ginsenosides-Rb1 inhibits the hypertrophy of cardiomyocytes via Nitric oxide synthase/nitric oxide system

作　　者：孔宏亮[1] 李占全[1] 袁龙[1] 

机构地区：[1]辽宁省人民医院心脏中心,沈阳110016

出　　处：《广东医学》2012年第2期167-169,共3页Guangdong Medical Journal

基　　金：辽宁省自然科学基金资助项目(编号:201102107)

摘　　要：目的在血管紧张素Ⅱ(AngⅡ,CoCl2)诱导乳鼠心肌细胞肥大基础上,探讨人参皂苷Rb1(ginsen-oside Rb1,Gs-Rb1)是否可通过一氧化氮合酶/一氧化氮(NOS/NO)系统减轻心肌细胞肥大。方法将乳鼠心肌细胞随机分为对照组、AngⅡ组、Gs-Rb1组、Gs-Rb1+L-NAME(NOS抑制剂)组、Gs-Rb1+AngⅡ+L-NAME组,AngⅡ、L-NAME和Gs-Rb1浓度分别是10μmol/L、1 mmol/L和200μmol/L;分别测定心肌细胞表面积、细胞培养液NO浓度和心肌细胞NOS活性。结果 (1)Gs-Rb1显著抑制AngⅡ所致的心肌细胞肥大(P=0.00),但不能使心肌细胞回复到正常大小;Gs-Rb1减小心肌细胞表面积的效应可被L-NAME显著抑制(P=0.00)。(2)AngⅡ降低心肌细胞NO浓度的效应可被Gs-Rb1显著抑制(P=0.00),但L-NAME可完全抑制Gs-Rb1对心肌细胞的NO分泌效应。(3)Gs-Rb1显著增加心肌细胞在AngⅡ干预时的NOS活性(P=0.00),该效应被L-NAME完全抑制。(4)心肌细胞表面积与NOS(r=0.59,P=0.00)、NO(r=0.62,P=0.00)均呈正相关。结论 Gs-Rb1显著抑制AngⅡ所致的心肌细胞肥大,此效应至少部分通过NOS/NO系统实现。Objective To investigate the inhibition of cardiomyocytes hypertrophy by Ginsenosides-Rb1（Gs-Rb1） via nitric oxide synthase/nitric oxide system（NOS/NO） on the basis of angiotensin Ⅱ（Ang Ⅱ） induced cardiomyocyte hypertrophy in vitro.Methods Neonatal rat cardiomyocytes were randomly divided into control group,Ang Ⅱ group,Gs-Rb1 group,Ang Ⅱ＋ L-NAME（nitric oxide synthase inhibitor） group,and Gs-Rb1＋Ang Ⅱ＋L-NAME group.The concentrations of Ang Ⅱ,Gs-Rb1 and L-NAME were 10 μmol/L,1 mmol/L and 200 μmol/L,respectively.The cell surface area,NO concentration and NOS activity were assayed.Results（1）Gs-Rb1 significantly inhibited cardiomyocyte hypertrophy（P=0.00） mediated by Ang Ⅱ,which was significantly inhibited with L-NAME（P=0.00）,though no complete recovery of cell surface area was observed.（2）The Ang Ⅱ-induced down-regulation of NO was significantly alleviated by Gs-Rb1（P=0.00）.However,this alleviation was completely eliminated by L-NAME.（3）Gs-Rb1 significantly increased NOS activity in Ang Ⅱ treated cardiomyocytes（P=0.00）,however,the effect was completely wiped off by L-NAME.（4）There were significant positive correlations between cell surface area and NOS activity（r=0.59,P=0.00）,and NO（r=0.62,P=0.00）.Conclusion Gs-Rb1 inhibits Ang Ⅱ-induced cardiomyocyte hypertrophy,which is partially mediated by NOS/NO system.

关 键 词：心肌细胞 血管紧张素Ⅱ 人参皂苷RB1 一氧化氮合酶 一氧化氮 

分 类 号：R542.2[医药卫生—心血管疾病]