复发性念珠菌性阴道炎患者阴道真菌菌群多样性研究  

A Study on Diversity of Vaginal Fungal Flora in Patients with Recurrent Vaginal Candidiasis

作　　者：郑能能[1] 刘玮[1] 漆丽娅[1] 陈晓新[1] 

机构地区：[1]浙江省立同德医院妇产科,浙江杭州310012

出　　处：《浙江预防医学》2012年第2期13-16,共4页Zhejiang Journal of Preventive Medicine

基　　金：浙江省医药卫生科学研究基金(2010KYA043)

摘　　要：目的分析复发性念珠菌性阴道炎(RVC)患者阴道真菌菌群的多样性。方法采用分离培养法和真菌18SrDNA序列分析方法,通过对RVC患者、单纯性念珠菌性阴道炎(SVC)患者和健康志愿者(H)阴道真菌18S rDNA序列进行克隆和测序,构建真菌18S rDNA克隆文库,建立系统发育树,获取各组研究对象阴道真菌菌群的结构和组成特征。结果 RVC患者、单纯性念珠菌性阴道炎患者和健康志愿者阴道标本中存在3个真菌类群14个属共19个种系型的真菌菌群,3个真菌类群分别为子蘘菌纲(89.96%)、担子菌纲(8.43%)和卵菌纲(1.61%)。健康志愿者、单纯性念珠菌性阴道炎患者和RVC患者组的阴道真菌菌落分别存在有8、11、15个种系型,只有4种真菌种系型共同存在于3组研究对象中。念珠菌属为健康志愿者、单纯性念珠菌性阴道炎患者和RVC患者组阴道真菌菌群的优势菌属,其阳性克隆子比例分别占41.18%、51.55%和46.20%;属于Uncultured eukaryote clone的克隆序列也在克隆文库中占据很大比例。结论人类阴道黏膜中存在丰富多样的真菌类群,RVC患者阴道真菌菌群分子生态结构发生明显改变,提示阴道真菌菌群紊乱可能与RVC发生发展相关。Objective To analyze the diversity of vaginal fungal flora in patients with recurrent vaginal candidiasis（RVC）.Methods Through isolation-culture method and 18S rDNA series analysis method,18S rDNA series of vaginal fungal flora were cloned and sequenced in healthy volunteers,simple and recurrent vaginal candidiasis patients,respectively.Then the structure and composition characteristics of individuals in different groups were obtained after constructing 18S rDNA cloning library and setting up phylogenetic tree.Results Three fungal groups（Ascomycotae 89.96%,Basidiomycetes 8.43%,Oomycetes 1.61%）with 14 genera including 19 phylotypes of fungi flora were found in vaginal specimens.The number of vaginal fungi flora which belonged to healthy volunteers,SVC and RVC patients was 8,11 and 15,respectively.Only 4 phylotypes found to coexist in all three groups.Candida spp.were the most advantage bacterium of three groups;the percent of positive cloning molecular was 41.18%,51.55 and 46.20%,respectively;uncultured eukaryote clones also constituted significant portions of the cloning library.Conclusion There is an abundant fungal flora in human vagina.Ecological structure of vaginal fungal flora appears to be various in patients with RVC,which indicate the close relationship between the alteration of ecological structure of fungal flora and the progression of RVC.

关 键 词：复发性念珠菌性阴道炎 真菌多样性 18S rDNA克隆文库 

分 类 号：R711.31[医药卫生—妇产科学]