珍稀林药植物齿瓣石斛芽增殖和快繁研究  

A Study on Bud Proliferation and Rapid Propagation of Rare Forest Medicine Plant of Dendrobium devonianum Paxt

作　　者：崔秋华[1] 孙永玉[1] 李昆[1] 周志美[2] 闫红[1] 黄传响 

机构地区：[1]中国林业科学研究院资源昆虫研究所,云南昆明650224 [2]云南省保山市森林资源管理总站,云南保山678000 [3]四川省攀枝花市林业局,四川攀枝花617000

出　　处：《江西农业大学学报》2011年第6期1139-1143,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：科技部农业成果转化基金项目(2008GB24320419)

摘　　要：以齿瓣石斛(Dendrobium devonianum Paxt)组培苗为外植体,对适合齿瓣石斛丛生芽增殖和生根的培养基进行筛选,并建立齿瓣石斛无性繁殖体系。研究结果表明,丛生芽增殖的最适培养基为MS+1.0 mg/L 6-BA+0.5 mg/L NAA+25 g/L蔗糖,在此培养基上,增殖系数高,幼苗生长状况好;继代苗在MS+0.3 mg/L NAA+20 g/L蔗糖的培养基上生根效果最好,根系长而粗壮;适于组培苗炼苗的最佳基质为甘蔗渣,两个月后,成活率达90%以上。其研究结果对野生石斛资源的保护和合理开发具有重要的意义,并为齿瓣石斛的规模化生产提供科学依据。By using thetissue culture seedlings of Dendrobium devonianum Paxt as explant to filter the best medium for bud proliferation and rooting,the vegetative propagation system of Dendrobium devonianum Paxt was established.The results showed that： the optimal medium for proliferation of multiple buds was MS＋6-BA 1.0 mg/L＋NAA 0.5 mg/L＋Sucrose 25 g/L.On this medium,the multiplication coefficient was high,and seedling grew well;subculture seedlings rooted well on the medium of MS ＋ NAA 0.3 mg/L ＋ sucrose 20 g/L,the roots were long and thick;the best hardened matrix for seedlings was sugar cane residue.Two months later,the survival rate was over 90%.The study has great significance in protection and rational exploitation of wild Dendrobium resources,and provides a scientific basis for large-scale production of Dendrobium devonianum Paxt.

关 键 词：齿瓣石斛 丛生芽增殖 生根 快繁研究 

分 类 号：Q943.1[生物学—植物学]