睾丸移植物间质细胞4种基因的表达分析  

A study of the expression of the four genes of leydig cells in allografted mouse testes

作　　者：徐安平[1] 夏勇[1] 杨会林[1] 龙霞[2] 李明华[2] 万汇涓[2] 于洁[2] 

机构地区：[1]北京大学深圳医院检验科,深圳518036 [2]北京大学深圳医院中心实验室

出　　处：《中国男科学杂志》2011年第12期3-7,共5页Chinese Journal of Andrology

基　　金：深圳市科技计划项目（201003098,201103032）

摘　　要：目的研究新生小鼠睾丸组织异体异位移植后，4种在睾丸间质细胞中起重要作用的基因表达情况，为异体异位睾丸组织移植模型用于科研及临床的可行性提供进一步实验数据。方法将162只1-2d昆明小鼠的睾丸移植到54只7-12w去势雄性免疫缺陷小鼠背部；在移植后9个时间段（3d和1-8w）取出移植物；选取4种在睾丸间质细胞中表达或高表达的基因313．hsd、1r、p450scc和star，采用聚合酶链反应，对发育不同阶段移植物中4种基因的表达情况进行分析，并与正常小鼠相应各年龄段睾丸中的基因表达情况相比较。结果在9个时间段取出的移植物中，所测定4种基因的表达趋势与在正常小鼠睾丸中所见基本相同。结论新生小鼠睾丸组织异体异位移植到免疫缺陷小鼠体内后，间质细胞4种受试基因的表达趋势与在正常小鼠中的表现基本相同。Objective To investigate the expression of the leydig cell genes and protein in allografted mouse testes and provides experimental data for the mouse leydig cell development in vitro. Methods Testes from 162 neonatal Kunming mice were grafted onto the back of 54 castrated BALB/c-nu/nu mice （7-12 week）. The grafts were then taken out at nine intervals （ 3 days, 1-8 weeks）. 3 β -hsd, lhr, p450scc and star, the four mouse leydig cell genes were detected with the reverse transcriptase-polymerase chain reaction. For comparison, the expression of these genes in the normal Kunming mice of relevant ages was also determined. Results In the grafts obtained from different experimental intervals, i.e..3 days and 1-8 weeks, the expression of 4 gene mRNAs showed similar trend with the ones in testes of the normal Kunming mice. Conclusion The expression of the four leydig cell genes in the grafts from neonatal mouse testis grafted into the immunodeficient mice is similar to that of the normal mice.

关 键 词：莱迪希细胞 睾丸/移植 基因表达 

分 类 号：Q291[生物学—细胞生物学]